Team:Freiburg/Project/Coop

Team Heidelberg

When we introduced Gibson cloning in our team we were facing quite some challenges but after several months of optimizing protocol and reagents and doing refinements we succeeded easily to have positive colonies carrying correctly assembled plasmids. We wanted to pass this laborious achievement and knowledge on to other iGEM teams. To help team Heidelberg establishing Gibson cloning for their project we decided to visit their laboratory.

For team Heidelberg it was very important to get Gibson cloning running as soon as possible. Their constructs are quite complex and time was running short. To demonstrate Gibson cloning we brought along a reporter plasmid of our project. This reporter plasmid consists of four parts: the backbone pBS1C3, a CMV promoter, an avGFP and a BGH terminator. Together we calculated the amounts of DNA fragments needed the Gibson reaction and pipetted the mix. After revealing some tricks and myths the Heidelberg iGEMs were able to yield E. coli carrying the reporter plasmid. To verify the functionality of the construct they checked for fluorescence and were happy to see green color.

To top this collaboration off Team Heidelberg provided a CHO (Chinese Hamster Ovary) cell line, which we could manipulate by our uniCAS toolkit.

Besides spending time in their iGEM lab we were shown around on campus and in the city of Heidelberg. Finally, we all enjoyed a fabulous dinner in the heart of the historic city center.

Team Norwich

To help the iGEM team of Norwich we also cooperated with this team. Therefore we collected soil in the garden of one of our team members. We sent 15 g soil to NRP-UEA-Norwich, which they want to screen for novel antimycins.
By sending them probes we hope to support this team to successfully conduct their iGEM project.

The TU München iGEM Team in Freiburg

During summer time iGEM team München has been several times in Freiburg to work in the lab of Prof. Dr. Reski at their Physcofilter-Project. Using the opportunity, we swapped ideas on our iGEM projects, sponsoring and human practice and organized a big barbecue.