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Team:BostonU/QS
From 2013.igem.org
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<h6>New Level 0 Parts</h6> | <h6>New Level 0 Parts</h6> | ||
| - | <p><h7>We have successfully made three new parts while working on the QS project. <a href="http://parts.igem.org/Part:BBa_K1114205">CvI</a> and <a href="http://parts.igem.org/Part:BBa_K1114206">CviR</a> were submitted to the Registry as Level 0 MoClo Parts. The pVioA promoter was confirmed after the DNA submission deadline and was not included in our submission.</p | + | <p><h7>We have successfully made three new parts while working on the QS project. <a href="http://parts.igem.org/Part:BBa_K1114205">CvI</a> and <a href="http://parts.igem.org/Part:BBa_K1114206">CviR</a> were submitted to the Registry as Level 0 MoClo Parts. The pVioA promoter was confirmed after the DNA submission deadline and was not included in our submission.</p> |
| - | <p | + | <p>All three parts were sequence verified. Our next steps involve testing the CviI/CviR genes for function and checking them for cross-reactivity with LuxI/LuxR.</p></h7> |
<h6>pVioA as a Constitutive Promoter</h6> | <h6>pVioA as a Constitutive Promoter</h6> | ||
Revision as of 00:32, 28 September 2013
Quorum Sensing
Quorum sensing is a system that controls population density using stimulus and response. These types of systems can be commandeered for use in synthetic biology and re-engineering to control gene expression in genetic circuits that are implanted in bacteria. The bacteria utilize quorum sensing to coordinate gene expression. To do so, the bacteria produce and secrete signaling molecules. These bacteria have receptors that detect the signaling molecule. When the signaling molecule binds to the receptor, it induces gene expression. The presence of one or more proteins will induce a promoter that controls the production of signal proteins, such as fluorescent proteins, or of other functional genes to link synthetic genetic circuits together.
A LuxIR-type quorum sensing system has been detected in Chromobacterium violaceum (Stauff et al., 2011). The goal is to introduce a new LuxR/I-like quorum sensing system to synthetic biology via MoClo. Chromobacterium violaceum, a gram-negative bacteria found in flora from water and soil in tropical and subtropical regions, uses the CviR/I system. The CviR/I system is homologous to the LuxR/I system. This can be done by cloning and characterizing CviR/I and pVioA, a promoter with CviR binding site) into E.coli.
New Level 0 Parts
All three parts were sequence verified. Our next steps involve testing the CviI/CviR genes for function and checking them for cross-reactivity with LuxI/LuxR.
pVioA as a Constitutive Promoter
