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Team:MIT/Project
From 2013.igem.org
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<li>Demonstrated Acyl-TyA targeting proteins to the cell membrane and into exosomes | <li>Demonstrated Acyl-TyA targeting proteins to the cell membrane and into exosomes | ||
<li>Designed a number of reporter constructs to assay for our signals:<br> | <li>Designed a number of reporter constructs to assay for our signals:<br> | ||
| - | + | <li> rtTA3 | |
| - |     <li> L7Ae | + | <li> L7Ae |
| - |     <li> Cas9-VP16 | + | <li> Cas9-VP16 |
| - |     <li> Cas9-Split Venus | + | <li> Cas9-Split Venus |
| - |     <li> Cre Recombinase | + | <li> Cre Recombinase |
Designed Acyl-TyA fusion proteins with our signals: | Designed Acyl-TyA fusion proteins with our signals: | ||
rtTA3 | rtTA3 | ||
Revision as of 02:46, 28 September 2013
The MIT iGEM team sought to create a new mode of engineered intercellular communication for use in <> by modifying the contents of existing exosomes through the use of the protein tag Acyl-TyA. We built on existing research targeting proteins to exosomes to enable intercellular communication by targeting signal proteins into exosomes and into HEK 293 receiver cells.
Over the past few months we have:
Demonstrated Acyl-TyA targeting proteins to the cell membrane and into exosomes
Designed a number of reporter constructs to assay for our signals:
rtTA3
L7Ae
Cas9-VP16
Cas9-Split Venus
Cre Recombinase
Designed Acyl-TyA fusion proteins with our signals:
rtTA3
L7Ae
Cas9-VP16
Cas9-Split Venus
Cre Recombinase
Demonstrated native exosomal microRNA repression with isolated exosomes and Jukat/HEK293 coculture experiments.
Demonstrated activation of a reporter using the trans activator Cas9-VP16.
Demonstrated DNA sensing using Cas9-Split Venus reconstitution.
Over the past few months we have:
