Team:MIT/Project

From 2013.igem.org

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<li> Cre Recombinase
<li> Cre Recombinase
</ul>
</ul>
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Designed Acyl-TyA fusion proteins with our signals:
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<li>Designed Acyl-TyA fusion proteins with our signals:
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rtTA3
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<ul>
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L7Ae
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<li> rtTA3
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Cas9-VP16
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<li> L7Ae
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Cas9-Split Venus
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<li> Cas9-VP16
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Cre Recombinase
+
<li> Cas9-Split Venus
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Demonstrated native exosomal microRNA repression with isolated exosomes and Jukat/HEK293 coculture experiments.
+
<li> Cre Recombinase
-
Demonstrated activation of a reporter using the trans activator Cas9-VP16.
+
</ul>
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Demonstrated DNA sensing using Cas9-Split Venus reconstitution.  
+
<li>Demonstrated native exosomal microRNA repression with isolated exosomes and Jukat/HEK293 coculture experiments.
 +
<li>Demonstrated activation of a reporter using the trans activator Cas9-VP16.
 +
<li>Demonstrated DNA sensing using Cas9-Split Venus reconstitution.  

Revision as of 03:16, 28 September 2013

iGEM 2012

Overview

  • Project Overview

miRNA Signal

  • Overview
  • siRNA Characterization
  • Exosome Isolation and Co-Culturing
  • Cell-Cell Co-Culturing

Protein Signals

  • Overview
  • GFP
  • rtTA3
  • Cre
  • L7Ae
  • Cas9-VP16

Novel DNA Sensor: Cas9 Split Venus Fusion

  • Overview
  • Leucine Zipper Fusion
  • DNA Sensing

Our BioBricks

  • Favorites
  • All BioBricks

Attributions

  • Attributions

The MIT iGEM team sought to create a new mode of engineered intercellular communication for use in <> by modifying the contents of existing exosomes through the use of the protein tag Acyl-TyA. We built on existing research targeting proteins to exosomes to enable intercellular communication by targeting signal proteins into exosomes and into HEK 293 receiver cells.

Over the past few months we have:

  • Demonstrated Acyl-TyA targeting proteins to the cell membrane and into exosomes
  • Designed a number of reporter constructs to assay for our signals:
    • rtTA3
    • L7Ae
    • Cas9-VP16
    • Cas9-Split Venus
    • Cre Recombinase
  • Designed Acyl-TyA fusion proteins with our signals:
    • rtTA3
    • L7Ae
    • Cas9-VP16
    • Cas9-Split Venus
    • Cre Recombinase
  • Demonstrated native exosomal microRNA repression with isolated exosomes and Jukat/HEK293 coculture experiments.
  • Demonstrated activation of a reporter using the trans activator Cas9-VP16.
  • Demonstrated DNA sensing using Cas9-Split Venus reconstitution.

    • Retrieved from "http://2013.igem.org/Team:MIT/Project"