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Team:MIT/L7Ae
From 2013.igem.org
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<h1>L7Ae Characterization</h1> | <h1>L7Ae Characterization</h1> | ||
| - | <p>To demonstrate L7Ae mediated repression, we transfected constitutively L7Ae and constitutive 2x-kturn_eGFP. As we increased the amount of transfected L7Ae, we saw increased repression of GFP expression.</p> | + | <p>To demonstrate L7Ae mediated repression, we transfected constitutively L7Ae and constitutive 2x-kturn_eGFP. As we increased the amount of transfected L7Ae, we saw increased repression of GFP expression.*Note that there was a mistake made in this transfection. 5x the amount of GFP was transfected in the control well; however, L7Ae clearly represses eGFP expression</p> |
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<img src="https://static.igem.org/mediawiki/2013/a/a5/L7aeone.png" width="200" height="250"> | <img src="https://static.igem.org/mediawiki/2013/a/a5/L7aeone.png" width="200" height="250"> | ||
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Revision as of 04:04, 28 September 2013
Overview
L7Ae is an RNA binding protein that represses translation of the targeted transcript. Regulating gene expression on the translational level allows for faster repression of the desired construct than transcriptional repressors. L7Ae targets a specific sequence, called the 2x-kturn, on the 5’ end of the RNA. We use L7Ae to repress constitutive 2x-kturn_eGFP. L7Ae can be used to create more complex genetic circuits that are regulated on both the translational and transcriptional level. By targeting L7Ae to exosomes we create the opportunity for the creation of more intricate feedback looks in receiver cells.L7Ae Characterization
To demonstrate L7Ae mediated repression, we transfected constitutively L7Ae and constitutive 2x-kturn_eGFP. As we increased the amount of transfected L7Ae, we saw increased repression of GFP expression.*Note that there was a mistake made in this transfection. 5x the amount of GFP was transfected in the control well; however, L7Ae clearly represses eGFP expression
Acyl-TyA-L7Ae: Exosome Isolation and Co-Culturing
Acyl-TyA-L7Ae: Cell-Cell Co-Culturing
