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Team:DTU-Denmark/Notebook/26 July 2013
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| + | [[Team:DTU-Denmark/Experiment1a|Native anaerobic stability of nitrite in ''E. coli''|experiment]] in order to ensure that any produced nitrite from Mutant 1 will not be consumed by any of the pathways in native ''E. coli''. | ||
==Results== | ==Results== | ||
Revision as of 17:53, 28 September 2013
26 July 2013
Contents |
Lab 115
Main purpose
Investigate nitrite stability in anaerobic, untransformed E. coli
Who was in the lab
Helen, Kasia
Procedure
Following the Native anaerobic stability of nitrite in E. coli|experiment in order to ensure that any produced nitrite from Mutant 1 will not be consumed by any of the pathways in native E. coli.
Results
Conclusion
Lab 208
Main purpose
- miniprep of TAT, Sec, and Nir for sequencing
- LB medium preparation (2L)
- Pick up new PAO1 cultures
- Make new colony PCR to get Nir from the newly acquired colonies.
Who was in the lab
Kristian, Julia
Procedure
PCRs:
- pZA21::RFP with primer pair 13 58C and 3:00.
- araBAD from K808000 with primer pair 12 61C and 1:00
- Colony PCR with first PAO1 colony but with a gradient of concentrations from 1x to 500x
- Colony PCR with the 4 new plates of PAO1, concentration x1
Results
See tomorrows gel.
Conclusion
Nothing to conclude from today's work.
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