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Team:DTU-Denmark/Notebook/26 July 2013
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[[Team:DTU-Denmark/Experiment1a|Native anaerobic stability of nitrite in ''E. coli'']] in order to ensure that any produced nitrite from Mutant 1 will not be consumed by any of the pathways in native ''E. coli''. | [[Team:DTU-Denmark/Experiment1a|Native anaerobic stability of nitrite in ''E. coli'']] in order to ensure that any produced nitrite from Mutant 1 will not be consumed by any of the pathways in native ''E. coli''. | ||
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=Lab 208= | =Lab 208= | ||
Revision as of 17:56, 28 September 2013
26 July 2013
Contents |
Lab 115
Main purpose
Investigate nitrite stability in anaerobic, untransformed E. coli
Who was in the lab
Helen, Kasia
Procedure
Following the experiment Native anaerobic stability of nitrite in E. coli in order to ensure that any produced nitrite from Mutant 1 will not be consumed by any of the pathways in native E. coli.
The results and the conclusion are shown in
Lab 208
Main purpose
- miniprep of TAT, Sec, and Nir for sequencing
- LB medium preparation (2L)
- Pick up new PAO1 cultures
- Make new colony PCR to get Nir from the newly acquired colonies.
Who was in the lab
Kristian, Julia
Procedure
PCRs:
- pZA21::RFP with primer pair 13 58C and 3:00.
- araBAD from K808000 with primer pair 12 61C and 1:00
- Colony PCR with first PAO1 colony but with a gradient of concentrations from 1x to 500x
- Colony PCR with the 4 new plates of PAO1, concentration x1
Results
See tomorrows gel.
Conclusion
Nothing to conclude from today's work.
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