Team:DTU-Denmark/Notebook/26 July 2013

From 2013.igem.org

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==Procedure==
==Procedure==
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Following [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_1a| Experiment 1a]]
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Following [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_1a| Experiment 1a]] in order to ensure that any produced nitrite from Mutant 1 will not be consumed by any of the pathways in native ''E. coli''.
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in order to ensure that any produced nitrite from Mutant 1 will not be consumed by any of the pathways in native ''E. coli''.
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The results and the conclusion are shown in [[Team:DTU-Denmark/Experiment1a|Native anaerobic stability of nitrite in ''E. coli'']].
The results and the conclusion are shown in [[Team:DTU-Denmark/Experiment1a|Native anaerobic stability of nitrite in ''E. coli'']].

Revision as of 17:57, 28 September 2013

26 July 2013

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Contents

Lab 115


Main purpose

Investigate nitrite stability in anaerobic, untransformed E. coli

Who was in the lab


Helen, Kasia

Procedure

Following Experiment 1a in order to ensure that any produced nitrite from Mutant 1 will not be consumed by any of the pathways in native E. coli.

The results and the conclusion are shown in Native anaerobic stability of nitrite in E. coli.

Lab 208


Main purpose


  • miniprep of TAT, Sec, and Nir for sequencing
  • LB medium preparation (2L)
  • Pick up new PAO1 cultures
  • Make new colony PCR to get Nir from the newly acquired colonies.

Who was in the lab


Kristian, Julia

Procedure


PCRs:

  • pZA21::RFP with primer pair 13 58C and 3:00.
  • araBAD from K808000 with primer pair 12 61C and 1:00
  • Colony PCR with first PAO1 colony but with a gradient of concentrations from 1x to 500x
  • Colony PCR with the 4 new plates of PAO1, concentration x1

Results


See tomorrows gel.

Conclusion


Nothing to conclude from today's work.

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