GigiGoesGugu (Talk | contribs) (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP'' <BR> I did a 50ul PCR reaction of the Backbone and...") |
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Sensing <BR> | Sensing <BR> | ||
| - | '''PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP'' <BR> | + | '''PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP''' <BR> |
I did a 50ul PCR reaction of the Backbone and insert of the effector constructs. I again used a program where the first ten cylces are 10C° bellow the norma annealing temperature. The Gel electrophoresis showed that there was some sort of contamination, proably in the Primer Mixes. So I did the primer mixes again. | I did a 50ul PCR reaction of the Backbone and insert of the effector constructs. I again used a program where the first ten cylces are 10C° bellow the norma annealing temperature. The Gel electrophoresis showed that there was some sort of contamination, proably in the Primer Mixes. So I did the primer mixes again. | ||
Sensing
PCR of the effector Backbones and the effector insert MMP2 and MMP9 without GFP
I did a 50ul PCR reaction of the Backbone and insert of the effector constructs. I again used a program where the first ten cylces are 10C° bellow the norma annealing temperature. The Gel electrophoresis showed that there was some sort of contamination, proably in the Primer Mixes. So I did the primer mixes again.
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