GigiGoesGugu (Talk | contribs) (Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''Measuring GFP expression''' <BR> Again I tried to induce GFP expression in the bacteria containing either the hya or the cad promote...") |
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Again I tried to induce GFP expression in the bacteria containing either the hya or the cad promoter. | Again I tried to induce GFP expression in the bacteria containing either the hya or the cad promoter. | ||
This time I washed and dilluted the cells before putting them in the different media. I also made three replicates of each medium, i.e. three wells with pH 5.5, three well with pH 7, etc. | This time I washed and dilluted the cells before putting them in the different media. I also made three replicates of each medium, i.e. three wells with pH 5.5, three well with pH 7, etc. | ||
| - | + | The absorbance was measured in a plate reader during 18h, a measurement was taken every 30 minutes. | |
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Sensing
Measuring GFP expression
Again I tried to induce GFP expression in the bacteria containing either the hya or the cad promoter.
This time I washed and dilluted the cells before putting them in the different media. I also made three replicates of each medium, i.e. three wells with pH 5.5, three well with pH 7, etc.
The absorbance was measured in a plate reader during 18h, a measurement was taken every 30 minutes.
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