Team:IIT Madras/Notebook
From 2013.igem.org
(Difference between revisions)
(→Protocols) |
(→Protocols) |
||
Line 278: | Line 278: | ||
---- | ---- | ||
(a) Added 1-2µl of DNA to competent cell. | (a) Added 1-2µl of DNA to competent cell. | ||
- | (b) Kept on ice for 30 minutes. | + | |(b) Kept on ice for 30 minutes. |
- | (c) Heat Shock at | + | |(c) Heat Shock at 42°C for 90 sec. |
+ | |(d) K | ||
== Phase III == | == Phase III == |
Revision as of 18:31, 6 July 2013
Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
---|
Contents |
Phase I : Ideation
Phase II : Experimentation
e-Notebook
May 13 | June 13 | July 13 | ||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
S | M | T | W | T | F | S | S | M | T | W | T | F | S | S | M | T | W | T | F | S | ||||||||||||||
1 | 2 | 3 | 4 | 1 | 1 | 2 | 3 | 4 | 5 | 6 | ||||||||||||||||||||||||
5 | 6 | 7 | 8 | 9 | 10 | 11 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | ||||||||||||||
6 | 7 | 8 | 9 | 10 | 11 | 12 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | ||||||||||||||
13 | 14 | 15 | 16 | 17 | 18 | 19 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | ||||||||||||||
20 | 21 | 22 | 23 | 24 | 25 | 26 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 28 | 29 | 30 | 31 | |||||||||||||||||
27 | 28 | 29 | 30 | 31 | 30 |
Experimental results
Protocols
1. Transformation:
(a) Added 1-2µl of DNA to competent cell. |(b) Kept on ice for 30 minutes. |(c) Heat Shock at 42°C for 90 sec. |(d) K
Phase III
Troubleshooting
Discussions
This page is under construction. For more information contact Rohan or Aman.