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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
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'''Cell Surface Display''' | '''Cell Surface Display''' | ||
''PCR optimisation:'' the previour PCR we did to amplify ''pINP_construct'' was not optimal. We thus did another gradient PCR of the same plasmid with 5% DMSO at 74, 76 and 78°C. | ''PCR optimisation:'' the previour PCR we did to amplify ''pINP_construct'' was not optimal. We thus did another gradient PCR of the same plasmid with 5% DMSO at 74, 76 and 78°C. | ||
A 0.8% Electrophoresis gel was performed to verify the reation's products. | A 0.8% Electrophoresis gel was performed to verify the reation's products. | ||
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| + | '''Nanoparticles''' | ||
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| + | Make nanoparticles : try 1 (added the cargo molecule (food dye) to GPs and left for stirring (for 3 days)). | ||
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{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Cell Surface Display
PCR optimisation: the previour PCR we did to amplify pINP_construct was not optimal. We thus did another gradient PCR of the same plasmid with 5% DMSO at 74, 76 and 78°C. A 0.8% Electrophoresis gel was performed to verify the reation's products.
Nanoparticles
Make nanoparticles : try 1 (added the cargo molecule (food dye) to GPs and left for stirring (for 3 days)).
"
