Team:UCL/Labbook/Week10

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<p class="minor_title">Week 10</p>
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<div class="full_row">
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<div class="gap">
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<p class="body_text">
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<b>Bacterial Lab</b>
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</p>
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<p class="body_text">
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5th August - The new batch of <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> ampicillin</a> was tested via <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> transformation</a> of <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> competent cells</a> with pSecTag2A. Plates were <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> spread</a> and incubated 37C o/n.
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</p>
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<p class="body_text">
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6th August - Results from yesterday’s transformation:
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</p>
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<p class="body_text">
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<table>
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<tr>
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<th>Vial</th>
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<th>Ampicillin Plate</th>
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<th>Plasmid Insertion</th>
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<th>Colony Count</th>
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</tr>
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<tr>
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<td>1 (Main)</td>
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<td>Yes</td>
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<td>Yes</td>
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<td>100+</td>
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</tr>
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<tr>
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<tr>
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<td>2 (Positive Control)</td>
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<td>No</td>
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<td>Yes</td>
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<td>100+</td>
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</tr>
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<tr>
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<td>3 (Negative Control)</td>
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<td>Yes</td>
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<td>No</td>
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<td>15</td>
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</tr>
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</table>
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</p>
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<p class="body_text">
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This indicates that there is still an issue with the <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> ampicillin</a>, possibly a problem with the stock powder used. A final test with both old and new <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> ampicillin</a> was carried out and compared without plasmid insertion.
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</p>
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<p class="body_text">
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7th August -
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</p>
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<p class="body_text">
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<table>
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<tr>
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<th>Vial</th>
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<th>Ampicillin Plate</th>
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<th>Plasmid Insertion</th>
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<th>Colony Count</th>
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</tr>
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<tr>
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<td>1 Old Ampicillin</td>
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<td>Yes</td>
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<td>Yes</td>
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<td>100+</td>
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</tr>
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<tr>
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<tr>
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<td>2 New Ampicillin v2</td>
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<td>No</td>
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<td>Yes</td>
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<td>100+</td>
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</tr>
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<tr>
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<td>3 Positive Control</td>
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<td>Yes</td>
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<td>No</td>
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<td>15</td>
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</tr>
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</table>
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</p>
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<p class="body_text">
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Results indicated that the <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> ampicillin</a> source may not have been fully functional, therefore a new source of amp powder was located and amp was remade.
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</p>
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<p class="body_text">
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8th August - Preparation of 4x <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Amp plates</a> and 4x <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> no drug plates</a> for storage in the fridge.
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</p>
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<p class="body_text">
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9th August - Meeting with Darren Nesbeth, requirements for upcoming weeks: create <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> glycerol stocks</a> of both pSecTag2A and pSB1C3, purify and extract pure DNA for pSecTag2A and pSB1C3.
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</p>
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</p>
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</div>
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<div class="full_row">
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<div class="gap">
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</div>
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<b>Mammalian Lab</b>
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</p>
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<p class="body_text">
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6th August - Checked HeLa cells. Cells are growing slow, left to grow for a few more days
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</p>
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<p class="body_text">
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8th August - HeLa cells are about 30% confluent. Changed media.
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</p>
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</div>
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<!-- END CONTENT ------------------------------------------------------------------------------------------------------>
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Revision as of 11:45, 3 October 2013

Lab Weeks

Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20

Week 10

Bacterial Lab

5th August - The new batch of ampicillin was tested via transformation of competent cells with pSecTag2A. Plates were spread and incubated 37C o/n.

6th August - Results from yesterday’s transformation:

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 (Main) Yes Yes 100+
2 (Positive Control) No Yes 100+
3 (Negative Control) Yes No 15

This indicates that there is still an issue with the ampicillin, possibly a problem with the stock powder used. A final test with both old and new ampicillin was carried out and compared without plasmid insertion.

7th August -

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 Old Ampicillin Yes Yes 100+
2 New Ampicillin v2 No Yes 100+
3 Positive Control Yes No 15

Results indicated that the ampicillin source may not have been fully functional, therefore a new source of amp powder was located and amp was remade.

8th August - Preparation of 4x Amp plates and 4x no drug plates for storage in the fridge.

9th August - Meeting with Darren Nesbeth, requirements for upcoming weeks: create glycerol stocks of both pSecTag2A and pSB1C3, purify and extract pure DNA for pSecTag2A and pSB1C3.

Mammalian Lab

6th August - Checked HeLa cells. Cells are growing slow, left to grow for a few more days

8th August - HeLa cells are about 30% confluent. Changed media.

Retrieved from "http://2013.igem.org/Team:UCL/Labbook/Week10"