Team:SDU-Denmark/Tour41

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Revision as of 12:36, 3 October 2013

Bacteriorganic Rubber

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You really should use our interactive wiki tour instead. :)
Don't worry, you won't miss out on anything. Everything below is part of the tour as well.

Looking for a specific page? Below is a direct shortcut to all our wiki-pages.

Introduction

Rubber issue

Production system

Process

Results

Future

Complete Journal

Important dates & Protocols

"If it is not documented, it doesn’t exist." - Louis Fried

Obviously, Louis Fried was wrong - and luckily so, we might add! But it is true that if it is not documented, we don't know whether it exist. So please, click through the tabs below and catch a quick glimpse of our most treasured moments of the summer. We kept a calender of special events since the start of the project. And if you have the yearning (particularly after reading our Results), open up our thorough protocols below.

The very beginning

28.02.2013 - Assembly of the team
08.03.2013 - 10.03.2013 - Teambuilding: iGEM crash course (DTU)
21.03.2013 - First meeting: get to know each other
26.03.2013 - Two new members of the team (Mattias and Sofie)
April + start of May - Brainstorming
08.05.2013 - Determination of project
11.07.2013 - One new member of the group (Nicky)

Social events

April
29.04.2013 - Social dinner and introduction to wiki design
July
11.07.2013 - Barbeque dinner
17.07.2013 - Tour des chambres
August
08.08.2013 - Squash tournament
22.08.2013 - Cabaret show, HCA festival 2013
31.08.2013 - 01.09.2013 - DK meetup
September
04.09.2013 - Supervisor Ann makes dinner for responsibility delegation meeting
25.09.2013 - Dinner after status meeting
26.09.2013 - Dinner with previous SDU iGEM teams

Lab successes

July
21.07.2013 - pSB1C3-Pcon-dxs(B. subtilis)-amilCP
25.07.2013 - B.sub DXS biobrick
25.07.2013 - E.coli IspG biobrick
29.07.2013 - pSB1C3-Plac-dxs(B. sub)-linker-GFP
31.07.2013 - pSB1C3-Plac-dxs(E. coli)-linker-GFP
August
01.08.2013 - We realized LacI should be overexpressed in order to get repression of the lac promoter
01.08.2013 - Sequencing of E. coli dxs standard part
05.08.2013 - pSB1C3-Plac-dxs(B. sub)
19.08.2013 - HRT2 biobrick
19.08.2013 - pSB1C3-Plac-dxs(E. coli)
19.08.2013 - pSB1C3-Pcon-lacI:LVA-term
17.09.2013 - LacI BioBrick
17.08.2013 - pSB1C3-Pcon-araC-term
21.08.2013 - pSB1C3-lacI:LVA-Plac-dxs(B. sub)
23.08.2013 - pSB1C3-lacI:LVA-Plac-dxs(B. sub)-linker-GFP
24.08.2013 - Characterization of lacI:LVA function
24.08.2013 - Confirmation that we could control the expression of Dxs with lacI:LVA using IPTG
28.08.2013 - pSB1C3-lacI:LVA-Plac-dxs(E. coli)
30.08.2013 - pSB1C3-Para-HRT2-(FLAG)
30.08.2013 - Growth experiment for MG1655/pSB1C3-lacI:LVA-Plac-dxs(B.sub)+/-GFP
September
13.09.2013 - pSB1C3-araC-Para-HRT2-(FLAG)
17.09.2013 - pSB1C3-lacI-Plac-dxs(B. sub)-linker-GFP
17.09.2013 - Characterized device with lacI and found that it functions better than lacI:LVA
20.09.2013 - Growth experiment for MG1655/pSB1C3-lacI-Plac-dxs(B.sub)
21.09.2013 - pSB1K3-araC-Para-HRT2-(FLAG)
21.09.2013 - Growth experiment for MG1655/pSB1C3-lacI-Plac-dxs(B.sub)-GFP
22.09.2013 - pSB1C3-lacI-Plac-dxs(B. sub)
October
01.10.2013 - Characterization of araC function
02.10.2013 - Confirmation that we could induce and control the expression of HRT2

iGEM deadlines

August
09.08.2013 - Team project description due
30.08.2013 - Track selection
30.08.2013 - Project title
September
23.09.2013 - BioBricks Part DNA shipped to iGEM HQ
25.09.2013 - BioBricks Part DNA at the Registry (Received at iGEM)
October
04.10.2013 - Wiki freeze
11.10.2013 - 13.10.2013 - Regional Jamboree

When working together in a large group, particularly on such a large project, it is simply impossible for anyone to maintain an overview over the entire project in real time. That is why it is important to keep protocols of every part of the project, to document every experiment that is performed. This allows each team member to backtrack and to resolve any issues with (or mistakes made in) previous experiments. Countless digestions and hundreds of PCR reaction were done, and the explicit protocols established which setups were effective, and which were not. Our iGEM-team has kept protocols since the dawn of the project, and they have proven to be an invaluable resource, which saved us quite a bit of time. To ensure the consistency of execution, each experiment was done by following one of our Standard Operating Procedures, which can be found under "SOPs".

Click on the project title below to expand the window and get an overview of the protocols for each project part. Each protocol name is a link to a pdf file that will open in a new window.

Primer design

Biobricks

Devices

Reporter systems

Characterization

@@ Line 37: / Line 37: @@
    <div class="accordionTitel">Social events</div>
-   <div class="pane" style="height:170px;" >
+   <div class="pane" style="height:240px;" >
+<b>April</b><br>
 .04.2013 - Social dinner and introduction to wiki design <br>
+<b>July</b><br>
 .07.2013 - Barbeque dinner <br>
 .07.2013 - Tour des chambres <br>
+<b>August</b><br>
 .08.2013 - Squash tournament <br>
 .08.2013 - Cabaret show, HCA festival 2013 <br>
 .08.2013 - 01.09.2013 - DK meetup <br>
+<b>September</b><br>
 .09.2013 - Supervisor Ann makes dinner for responsibility delegation meeting <br>
 .09.2013 - Dinner after status meeting <br>
@@ Line 56: / Line 59: @@
    <div class="accordionTitel">Lab successes</div>
-   <div class="pane" style="height:550px;" >
+   <div class="pane" style="height:600px;" >
+<b>July</b><br>
 .07.2013 - pSB1C3-Pcon-dxs(B. subtilis)-amilCP <br>
 .07.2013 - B.sub DXS biobrick  <br>
@@ Line 63: / Line 67: @@
 .07.2013 - pSB1C3-Plac-dxs(B. sub)-linker-GFP <br>
 .07.2013 - pSB1C3-Plac-dxs(E. coli)-linker-GFP <br>
+<b>August</b><br>
 .08.2013 - We realized LacI should be overexpressed in order to get repression of the lac promoter<br>
 .08.2013 - Sequencing of E. coli dxs standard part <br>
@@ Line 78: / Line 83: @@
 .08.2013 - pSB1C3-Para-HRT2-(FLAG)<br>
 .08.2013 - Growth experiment for MG1655/pSB1C3-lacI:LVA-Plac-dxs(B.sub)+/-GFP <br>
+<b>September</b><br>
 .09.2013 - pSB1C3-araC-Para-HRT2-(FLAG) <br>
 .09.2013 - pSB1C3-lacI-Plac-dxs(B. sub)-linker-GFP <br>
@@ Line 85: / Line 91: @@
 .09.2013 - Growth experiment for MG1655/pSB1C3-lacI-Plac-dxs(B.sub)-GFP <br>
 .09.2013 - pSB1C3-lacI-Plac-dxs(B. sub) <br>
+<b>October</b><br>
 .10.2013 - Characterization of araC function <br>
-.10.2013 - Confirmation that we could induce and control the expression of HRT2 with AraC using arabinose <br>
+.10.2013 - Confirmation that we could induce and control the expression of HRT2<br>
@@ Line 94: / Line 101: @@
    <div class="accordionTitel">iGEM deadlines</div>
    <div class="pane" style="height:150px;" >
+<b>August</b><br>
 .08.2013 - Team project description due <br>
 .08.2013 - Track selection <br>
 .08.2013 - Project title <br>
+<b>September</b><br>
 .09.2013 - BioBricks Part DNA shipped to iGEM HQ <br>
 .09.2013 - BioBricks Part DNA at the Registry (Received at iGEM) <br>
+<b>October</b><br>
 .10.2013 - Wiki freeze <br>
 .10.2013 - 13.10.2013 - Regional Jamboree <br>