Team:TU Darmstadt/protocols

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<br>
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<h2><font size="6" color="#F0F8FF" face="Arial regular">Protocols</font></h2>
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<p text-aligne:left style="margin-left:50px; margin-right:50px">
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The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project.
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<br>
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<font size="6" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:50px; margin-right:50px">
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In vivo methods
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<font size="3" color="#F0F8FF" face="Arial regular">
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<ul style="margin-left:50px; margin-right:50px; text-align:justify; ">
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<li> <a href="https://2013.igem.org/Team:TU_Darmstadt/protocols/Bacterial_cell_culture"><font size="3" color="#F0F8FF" face="Arial regular"><b>Bacterial cell culture</b></font></a></li>
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<li><a href="https://2013.igem.org/Team:TU_Darmstadt/protocols/Bacterial_transformation"><font size="3" color="#F0F8FF" face="Arial regular"><b> Bacterial transformation    </b></font></a> </li>
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<li> <a href="https://2013.igem.org/Team:TU_Darmstadt/protocols/Cell_counting/plating        "><font size="3" color="#F0F8FF" face="Arial regular"><b>Cell  counting/plating    </b></font></a></li>
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<li> <a href="https://2013.igem.org/Team:TU_Darmstadt/protocols/Chemically_competent_cells        "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Chemically competent cells    </b></font></a></li>
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<li> <a href="  https://2013.igem.org/Team:TU_Darmstadt/protocols/Colony_PCR        "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Colony PCR  </b></font></a></li>
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<li> <a href="  https://2013.igem.org/Team:TU_Darmstadt/protocols/Glycerine_stock      "><font size="3" color="#F0F8FF" face="Arial regular"><b>    Glycerine Stocks  </b></font></a></li>
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<li> <a href="  https://2013.igem.org/Team:TU_Darmstadt/protocols/Heat_Shock_Transformation      "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Heat Shock Transformation    </b></font></a></li>
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/protocols/Protein_Expression        "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Protein expression    </b></font></a></li>
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</ul>
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<font size="6" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:50px; margin-right:50px">
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In vitro methods
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<font size="5" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:50px; margin-right:50px">
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For DNA
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{| style="color:green;background:none;"
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</font>
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!align="center"|[[Team:TU_Darmstadt/labbook|<span style="color:white;font-size:160%;"> Labbook |</span>]]
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</p>
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!align="center"|[[Team:TU_Darmstadt/protocols|<span style="color:white;font-size:160%;"> Protocols |</span> ]]
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!align="center"|[[Team:TU_Darmstadt/materials|<span style="color:white;font-size:160%;"> Materials |</span>]]
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|}
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The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project. The corresponding materials can be found in the [https://2012.igem.org/Team:TU_Darmstadt/materials materials section].
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<font size="3" color="#F0F8FF" face="Arial regular">
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<p text-aligne:left style="margin-left:50px; margin-right:50px">
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== In vivo ==
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<ul style="margin-left:50px; margin-right:50px; text-align:justify; ">
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=== [http://en.wikipedia.org/wiki/E._coli Escherichia coli] ===
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<li> <a href="  https://2013.igem.org/Team:TU_Darmstadt/protocols/Agarose_gel_electrophoresis      "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Agarose gel electrophoresis  </b></font></a></li>
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Bacterial_cell_culture Bacterial cell culture]
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<li> <a href="  https://2013.igem.org/Team:TU_Darmstadt/protocols/Dephosphorylation      "><font size="3" color="#F0F8FF" face="Arial regular"><b>  Dephosphorylation    </b></font></a></li>
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Bacterial_transformation Bacterial transformation]
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<li> <a href="  https://2013.igem.org/Team:TU_Darmstadt/protocols/DNA_Ligation      "><font size="3" color="#F0F8FF" face="Arial regular"><b>  DNA Ligation    </b></font></a></li>
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Cell_counting/plating Cell counting/plating]
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<li> <a href="https://2013.igem.org/Team:TU_Darmstadt/protocols/DNA_Quantification        "><font size="3" color="#F0F8FF" face="Arial regular"><b>  DNA Quantification with NanoDrop    </b></font></a></li>
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Chemically_competent_cells Chemically competent cells]
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<li> <a href="https://2013.igem.org/Team:TU_Darmstadt/protocols/InFusion        "><font size="3" color="#F0F8FF" face="Arial regular"><b> InFusion cloning    </b></font></a></li>
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Colony_PCR Colony PCR]
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/protocols/PCR       "><font size="3" color="#F0F8FF" face="Arial regular"><b> PCR    </b></font></a></li>
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Glycerine_stock Glycerine Stocks]
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<li> <a href="  https://2013.igem.org/Team:TU_Darmstadt/protocols/Miniprep      "><font size="3" color="#F0F8FF" face="Arial regular"><b>  PureYield Plasmid Miniprep System    </b></font></a></li>
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Heat_Shock_Transformation Heat Shock Transformation]
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<li> <a href="  https://2013.igem.org/Team:TU_Darmstadt/protocols/Restriction_digest      "><font size="3" color="#F0F8FF" face="Arial regular"><b> Restriction digest    </b></font></a></li>
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Protein_Expression Protein expression]
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<li> <a href=" http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag        "><font size="3" color="#F0F8FF" face="Arial regular"><b> Sequencing of DNA    </b></font></a></li>
 +
<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/protocols/Purification        "><font size="3" color="#F0F8FF" face="Arial regular"><b> Wizard SV Gel and PCR Clean-Up System    </b></font></a></li>
 +
</ul>
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<br>
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== In vitro ==
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<font size="5" color="#F0F8FF" face="Arial regular">
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=== [http://en.wikipedia.org/wiki/DNA DNA] ===
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<p text-aligne:left style="margin-left:50px; margin-right:50px">
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
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-
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Dephosphorylation Dephosphorylation]
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/DNA_Ligation DNA Ligation]
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-
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/DNA_Quantification DNA Quantification with NanoDrop]
+
-
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/InFusion InFusion cloning]
+
-
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/PCR PCR]
+
-
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Miniprep PureYield Plasmid Miniprep System]
+
-
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Restriction_digest Restriction digest]
+
-
* [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA]
+
-
* [https://2013.igem.org/Team:TU_Darmstadt/protocols/Purification Wizard SV Gel and PCR Clean-Up System]
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-
=== [http://en.wikipedia.org/wiki/Protein Protein] ===
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For Proteins
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/SDS-Page SDS-Page]
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</font>
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* [https://2013.igem.org/Team:TU_Darmstadt/protocols/SDS-Page Spectra measurement]
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</p>
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<ul style="margin-left:50px; margin-right:50px; text-align:justify; ">
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<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/protocols/SDS-Page       "><font size="3" color="#F0F8FF" face="Arial regular"><b> SDS-Page     </b></font></a></li>
 +
<li> <a href=" https://2013.igem.org/Team:TU_Darmstadt/protocols/SDS-Page       "><font size="3" color="#F0F8FF" face="Arial regular"><b> Spectra measurement     </b></font></a></li>
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</ul>
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<br><br>

Revision as of 21:56, 3 October 2013




Protocols

The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project.

In vivo methods

In vitro methods

For DNA


For Proteins



Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/protocols"