Team:TU Darmstadt/materials/Phusion
From 2013.igem.org
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Latest revision as of 17:47, 3 October 2013
Labbook | | Protocols | | Materials | |
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Contents |
Phusion
About
Phusion is a Pyrococcus Pfu-like DNA polymerase with a dobule-strand DNA binding domain, resulting in an enhanced processivity and higher activity than other available DNA polymerases like taq and creates blunt ends.
Working conditions
- 98°C denaturation temperature
- ~30 s/kb extension velocity (at 72°C)
- (TM-3)°C=TA
References
- http://www.neb.com/products/m0530-phusion-high-fidelity-dna-polymerase