"
Team:Paris Bettencourt/Notebook/Trojan Horse/Wednesday 4th September.html
From 2013.igem.org
| Line 7: | Line 7: | ||
<p><b><em> | <p><b><em> | ||
<!-- === Modify from here === --> | <!-- === Modify from here === --> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/4/45/ParisB2013TH_4_09.png" width=" | + | <img src="https://static.igem.org/mediawiki/2013/4/45/ParisB2013TH_4_09.png" width="400" height="100"> |
<!-- === To here === --> | <!-- === To here === --> | ||
</br></em></b></p> | </br></em></b></p> | ||
Revision as of 18:14, 3 October 2013
Trojan Horse
ASDF4th September
Results of silencing experiments
It works !
Miller assay
XXXXXX
Redo the silencing experiment
- Launch O/N culture of producing phages cells ; centrifuge ; take supernatant and filter it.
-diluting 100x O/N of MGZ1, F+ -pACYc in LB-Cm (25 ug/ul)
- At OD 0,7 : 5 tubes. Put 900 uL of cells in each tube. Add in tubes respectively : nothing, 100uL phages of litmusGFP-LacZ1, litmusGFP-Cm1 & litmusGFP-Cm2
litmusGFP-lacZ1 & litmusGFP-lacZ2 are used as negative control.
- Incubate 45 minutes at 37°C
- Serial Dilute every tube until 10-5. Plate 100ul for each tube according to this dilution table
|
LB |
Cm 25 ug/ul |
Cm 125 ug/ul |
Cm 250ug/ul |
Cm 500 ug/ul |
|
|
non infected |
10^-4 |
10^-4 |
10^-4 |
10^-4 |
10^-4 |
|
sRNA anti lacz |
10^-4 |
10^-4 |
10^-3 |
10^-3 |
10^-3 |
|
sRNA Cm1 |
10^-4 |
10^-4 |
10^-3 |
10^-3 |
10^-3 |
|
sRNA Cm2 |
10^-4 |
10^-4 |
10^-3 |
10^-3 |
10^-3 |
|
sRNA Cm3 |
10^-4 |
10^-4 |
10^-3 |
10^-3 |
10^-3 |
|
sRNA Cm4 |
10^-4 |
10^-4 |
10^-3 |
10^-3 |
10^-3 |
