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Team:TU Darmstadt/protocols/InFusion
From 2013.igem.org
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<B> Materials<br></B> | <B> Materials<br></B> | ||
| + | 5X In-Fusion HD Enzyme Premix | ||
| + | <br>pUC19 Control Vector,linearized (50 ng/μl) | ||
| + | <br>2 kb Control Insert (40 ng/μl)<br> | ||
<br> | <br> | ||
<B> Equipment<br></B> | <B> Equipment<br></B> | ||
Revision as of 17:40, 4 October 2013
In Fusion
Materials
5X In-Fusion HD Enzyme Premix
pUC19 Control Vector,linearized (50 ng/μl)
2 kb Control Insert (40 ng/μl)
Equipment
In-Fusion® HD Cloning Kit
Micropipettes with sterile tips
Chemicals & consumables
Procedure
1.
2.
3.
4.
