"
Team:TU Darmstadt/protocols/PCR
From 2013.igem.org
(Difference between revisions)
| Line 162: | Line 162: | ||
<p text-aligne:left style="margin-left:50px; margin-right:50px"> | <p text-aligne:left style="margin-left:50px; margin-right:50px"> | ||
<B> Short report<br></B> | <B> Short report<br></B> | ||
| - | + | PCR (polymerase chain reaction) is a method for exponentially amplifying a fragment of DNA in vitro. | |
<B> Materials<br></B> | <B> Materials<br></B> | ||
<br> | <br> | ||
Revision as of 16:46, 4 October 2013
PCR
Short report
PCR (polymerase chain reaction) is a method for exponentially amplifying a fragment of DNA in vitro.
Materials
Equipment
Micropipettes with sterile tips
PCR machine
PCR tubes
Chemicals & consumables
DNA template
Nuclease-free water
Primer
Polymerase buffer
dNTP mix
DMSO
Procedure
This is a commonly used protocol thta we used for Taq-polymerase
PCR program (30 cycles)
initial denaturation 95°C, 60s
denaturation 95°C, 45s
annealing 54,5°C, 90s
elongation 72°C, 120s
final elongation 72°C, 120s
After worth run an analytic 1% agarose gel and purificate the product with Wizard SV Gel and PCR Clean-Up System (Promega)
