Team:TU Darmstadt/protocols/PCR

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<img alt="Problem" src="/wiki/images/6/66/Darmstadt_green_Problem.jpg" width="100" height="30"></a>
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<!-- PCR -->
<!-- PCR -->

Revision as of 23:46, 4 October 2013





PCR

Short report
 PCR (polymerase chain reaction) is a method for exponentially amplifying a fragment of DNA in vitro. Materials
Equipment
 Micropipettes with sterile tips
PCR machine
PCR tubes

Chemicals & consumables
DNA template
Nuclease-free water
Primer
Polymerase buffer
dNTP mix
DMSO

Procedure
 This is a commonly used protocol thta we used for Taq-polymerase
PCR program (30 cycles)
 initial denaturation 95°C, 60s
denaturation 95°C, 45s
annealing 54,5°C, 90s
elongation 72°C, 120s
final elongation 72°C, 120s

After worth run an analytic 1% agarose gel and purificate the product with Wizard SV Gel and PCR Clean-Up System (Promega)

Retrieved from "http://2013.igem.org/Team:TU_Darmstadt/protocols/PCR"