"
Team:TU Darmstadt/protocols/Glycerine stock
From 2013.igem.org
(Difference between revisions)
(Created page with "<html> <style type="text/css"> body { margin:0; padding:15px 0 0; background-color: #ececec; background-image:url("https://static.igem.org/mediawiki/2013/1/14/Background_(3000x180_Pi...") |
|||
| Line 111: | Line 111: | ||
z-index: 5; | z-index: 5; | ||
} | } | ||
| - | + | LI.list1 {list-style: none; color:white;} | |
| + | .blacktext {color:black} | ||
| + | p, ul { | ||
| + | padding: 0; | ||
| + | margin: 0; | ||
| + | } | ||
</style> | </style> | ||
| Line 159: | Line 164: | ||
<body> | <body> | ||
| - | <font size=" | + | <p text-aligne:left style="margin-left:50px; margin-right:50px"><font size="4.5" color="#F0F8FF" face="Arial regular"> |
| - | <p text-aligne:left style="margin-left: | + | <B> Procedure<br></B></font></p><br> |
| - | < | + | <p text-aligne:left style="margin-left:60px; margin-right:60px"><font size="3" color="#F0F8FF" face="Arial regular"> |
| - | In order to have a permanent culture of cells glycerine stocks can be made.< | + | In order to have a permanent culture of cells glycerine stocks can be made. |
| - | < | + | <div align="left" style="margin-left:30px; margin-right:50px"> |
| - | < | + | <ol> |
| - | + | <li>Add 200 µl of sterilized glycerine or DMSO to 800 µl cell culture and mix well.</li> | |
| - | + | <li>Freeze the stock at -20 °C.</li> | |
| - | <br> | + | </ol> |
| + | </div> | ||
| + | </font></p><br> | ||
Revision as of 21:31, 4 October 2013
Glycerine stock
Procedure
In order to have a permanent culture of cells glycerine stocks can be made.
