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Team:TU Darmstadt/materials/Media
From 2013.igem.org
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1.7-DYT (recipe for 500 mL) | 1.7-DYT (recipe for 500 mL) | ||
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<li>2.5 g NaCl</li> | <li>2.5 g NaCl</li> | ||
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<p text-aligne:left style="margin-left:0px; margin-right:50px"> | <p text-aligne:left style="margin-left:0px; margin-right:50px"> | ||
LB (recipe for 1000 mL) | LB (recipe for 1000 mL) | ||
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<li>10 g tryptone</li> | <li>10 g tryptone</li> | ||
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SOB (recipe for 1000 mL) | SOB (recipe for 1000 mL) | ||
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<li>0.186 g KCl</li> | <li>0.186 g KCl</li> | ||
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SOC (recipe for 1000 mL) | SOC (recipe for 1000 mL) | ||
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<li>980 mL SOB</li> | <li>980 mL SOB</li> | ||
Revision as of 20:53, 4 October 2013
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Media
1.7-DYT (recipe for 500 mL)
- 2.5 g NaCl
- 8.0 g tryptone
- 5.0 g yeast extract
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
LB (recipe for 1000 mL)
- 10 g tryptone
- 5 g yeast extract
- 10 g NaCl
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
- for plates: add 15g agar
SOB (recipe for 1000 mL)
- 0.186 g KCl
- 2.4 g MgSO4
- 0.584 g NaCl
- 20 g tryptone
- 5 g yeast extract
- add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
SOC (recipe for 1000 mL)
- 980 mL SOB
- 20 mL 1M Glucose
