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Team:TU Darmstadt/protocols/Protein Expression
From 2013.igem.org
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<br><br><br><br> | <br><br><br><br> | ||
<h2><font size="6" color="#F0F8FF" face="Arial regular">Protein Expression</font></h2> | <h2><font size="6" color="#F0F8FF" face="Arial regular">Protein Expression</font></h2> | ||
| + | <body> | ||
| - | + | <p text-aligne:left style="margin-left:50px; margin-right:50px"><font size="4.5" color="#F0F8FF" face="Arial regular"> | |
| - | <p text-aligne:left style="margin-left:50px; margin-right:50px"> | + | <B> Materials<br></B></font></p> |
| - | + | ||
<p text-aligne:left style="margin-left:60px; margin-right:50px"><font size="3" color="#F0F8FF" face="Arial regular"> | <p text-aligne:left style="margin-left:60px; margin-right:50px"><font size="3" color="#F0F8FF" face="Arial regular"> | ||
<br> | <br> | ||
| - | <B>Equipment | + | <B>Equipment<br></B> |
<div align="left" style="margin-left:60px; margin-right:50px"> | <div align="left" style="margin-left:60px; margin-right:50px"> | ||
<ul> | <ul> | ||
<li class=list1>- Incubation shaker</li> | <li class=list1>- Incubation shaker</li> | ||
| - | <li class=list1>- < | + | <li class=list1>- Photometer</li> |
| - | <li class=list1>- | + | </ul> |
| + | </div> | ||
| + | </font> | ||
| + | </p> | ||
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| + | <p text-aligne:left style="margin-left:60px; margin-right:50px"><font size="3" color="#F0F8FF" face="Arial regular"> | ||
| + | <br> | ||
| + | <B>Chemicals & consumables<br></B> | ||
| + | <div align="left" style="margin-left:60px; margin-right:50px"> | ||
| + | <ul> | ||
| + | <li class=list1>- E. coli BL21 DE3</li> | ||
| + | <li class=list1>- dYT media</li> | ||
<li class=list1>- IPTG</li> | <li class=list1>- IPTG</li> | ||
<li class=list1>- 100 ml and 3 l flasks</li> | <li class=list1>- 100 ml and 3 l flasks</li> | ||
| - | |||
<li class=list1>- ice</li> | <li class=list1>- ice</li> | ||
</ul> | </ul> | ||
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</font> | </font> | ||
</p> | </p> | ||
| - | + | <br> | |
<p text-aligne:left style="margin-left:50px; margin-right:50px"><font size="4.5" color="#F0F8FF" face="Arial regular"> | <p text-aligne:left style="margin-left:50px; margin-right:50px"><font size="4.5" color="#F0F8FF" face="Arial regular"> | ||
| - | <B> Procedure<br></B></font></p> | + | <B> Procedure<br></B></font></p><br> |
<p text-aligne:left style="margin-left:60px; margin-right:60px"><font size="3" color="#F0F8FF" face="Arial regular"> | <p text-aligne:left style="margin-left:60px; margin-right:60px"><font size="3" color="#F0F8FF" face="Arial regular"> | ||
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<div align="left" style="margin-left:30px; margin-right:50px"> | <div align="left" style="margin-left:30px; margin-right:50px"> | ||
<ol> | <ol> | ||
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<li>Inoculation of 50 mL DYT medium in the 100 mL flask with <i>E. coli</i> BL21 DE3 containing <a href=http://lucerna-chem.ch/shop/558271> pPR-IBA2 </a> | <li>Inoculation of 50 mL DYT medium in the 100 mL flask with <i>E. coli</i> BL21 DE3 containing <a href=http://lucerna-chem.ch/shop/558271> pPR-IBA2 </a> | ||
plasmid with the sequence for the respective part to be expressed.</li> | plasmid with the sequence for the respective part to be expressed.</li> | ||
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<li>Incubation of the cell suspension over night at 180 rpm at 30°C.</li> | <li>Incubation of the cell suspension over night at 180 rpm at 30°C.</li> | ||
</ol> | </ol> | ||
| + | </div> | ||
| + | </font></p> | ||
Revision as of 21:54, 4 October 2013
Protein Expression
Materials
Equipment
Chemicals & consumables
Procedure
