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Team:TU Darmstadt/protocols/PCR
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Revision as of 00:14, 5 October 2013
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PCR
Short report
PCR (polymerase chain reaction) is a method for exponentially amplifying a fragment of DNA in vitro.
Materials
Equipment
Micropipettes with sterile tips
PCR machine
PCR tubes
Chemicals & consumables
DNA template
Nuclease-free water
Primer
Polymerase buffer
dNTP mix
DMSO
Procedure
This is a commonly used protocol thta we used for Taq-polymerase
PCR program (30 cycles)
initial denaturation 95°C, 60s
denaturation 95°C, 45s
annealing 54,5°C, 90s
elongation 72°C, 120s
final elongation 72°C, 120s
After worth run an analytic 1% agarose gel and purificate the product with Wizard SV Gel and PCR Clean-Up System (Promega)
