User:ChristopherW

From 2013.igem.org

(Difference between revisions)
(Sample Output)
Line 31: Line 31:
|-
|-
|A (Ala)
|A (Ala)
-
|1 (12.50%)
+
|3 (8.57%)
|R (Arg)
|R (Arg)
|0 (0.00%)
|0 (0.00%)
Line 37: Line 37:
|0 (0.00%)
|0 (0.00%)
|D (Asp)
|D (Asp)
-
|1 (12.50%)
+
|2 (5.71%)
|C (Cys)
|C (Cys)
-
|0 (0.00%)
+
|1 (2.86%)
|Q (Gln)
|Q (Gln)
-
|0 (0.00%)
+
|2 (5.71%)
|E (Glu)
|E (Glu)
-
|0 (0.00%)
+
|1 (2.86%)
|G (Gly)
|G (Gly)
-
|0 (0.00%)
+
|1 (2.86%)
|H (His)
|H (His)
-
|0 (0.00%)
+
|1 (2.86%)
|-
|-
|K (Lys):
|K (Lys):
-
|2 (25.00%)
+
|2 (5.71%)
|M (Met)
|M (Met)
-
|0 (0.00%)
+
|2 (5.71%)
|F (Phe)
|F (Phe)
-
|0 (0.00%)
+
|2 (5.71%)
|P (Pro)
|P (Pro)
-
|0 (0.00%)
+
|2 (5.71%)
|S (Ser)
|S (Ser)
-
|1 (12.50%)
+
|3 (8.57%)
|T (Thr)
|T (Thr)
-
|0 (0.00%)
+
|3 (8.57%)
|W (Trp)
|W (Trp)
-
|1 (12.50%)
+
|2 (5.71%)
|Y (Tyr)
|Y (Tyr)
-
|0 (0.00%)
+
|2 (5.71%)
|V (Val)
|V (Val)
-
|0 (0.00%)
+
|2 (5.71%)
|-
|-
|colspan="3"|'''Amino acid counting:'''
|colspan="3"|'''Amino acid counting:'''
|colspan="3"|Total number of amino acids (aa):
|colspan="3"|Total number of amino acids (aa):
-
|colspan="2"|8
+
|colspan="2"|35
-
|colspan="3"|Number of positively charged aa:
+
|colspan="3"|Number of positively charged aa (Arg + Lys):
-
|colspan="2"|?9?
+
|colspan="2"|2
-
|colspan="3"|Number of negatively charged aa:
+
|colspan="3"|Number of negatively charged aa (Asp + Glu):
-
|colspan="2"|?9?
+
|colspan="2"|3
|-
|-
|colspan="3"|'''Biochemical parameters:'''
|colspan="3"|'''Biochemical parameters:'''
|colspan="3"|Molecular weight [Da]:
|colspan="3"|Molecular weight [Da]:
-
|colspan="2"|733.82
+
|colspan="2"|3613.05
|colspan="3"|Theoretical pI:
|colspan="3"|Theoretical pI:
-
|colspan="2"|?4?
+
|colspan="2"|5.38
|colspan="3"|Extinction coefficient:
|colspan="3"|Extinction coefficient:
|colspan="2"|?4?
|colspan="2"|?4?

Revision as of 20:27, 16 July 2013

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Sample Output

Automatically determined parameters using the [http://www.mediawiki.org/wiki/Table BioBrick-AutoAnnotator]
BioBrick: BBa_K1159000 Used open reading frame from position ?x? to ?y?.
A (Ala) 3 (8.57%) R (Arg) 0 (0.00%) N (Asn) 0 (0.00%) D (Asp) 2 (5.71%) C (Cys) 1 (2.86%) Q (Gln) 2 (5.71%) E (Glu) 1 (2.86%) G (Gly) 1 (2.86%) H (His) 1 (2.86%)
K (Lys): 2 (5.71%) M (Met) 2 (5.71%) F (Phe) 2 (5.71%) P (Pro) 2 (5.71%) S (Ser) 3 (8.57%) T (Thr) 3 (8.57%) W (Trp) 2 (5.71%) Y (Tyr) 2 (5.71%) V (Val) 2 (5.71%)
Amino acid counting: Total number of amino acids (aa): 35 Number of positively charged aa (Arg + Lys): 2 Number of negatively charged aa (Asp + Glu): 3
Biochemical parameters: Molecular weight [Da]: 3613.05 Theoretical pI: 5.38 Extinction coefficient: ?4?
Estimated half-life: Mammals: ?4 h? Yeast: ?4? E. coli: ?4?
Codon usage: Mammals: ?good? Yeast: ?Ok? E. coli: ?bad ?
RFC standard: This is a RFC 25 BioBrick, thus ATGGCCGGC and ACCGGT were added to the 5' and 3' ends.
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For information please read the description.