"
Team:BYU Provo/Notebook/CholeraDetection/Fallexp/Period3/Dailylog
From 2013.igem.org
| Line 36: | Line 36: | ||
<font size="4"> '''10/9/2013''' </font> | <font size="4"> '''10/9/2013''' </font> | ||
| + | Set up overnights of SdiA+/- strains for later infection with bacteriophage Lambda. Lysogens will plated next to Cholera to prove that SdiA is necessary for lytic induction of bacteriophage Lambda. | ||
| + | Begin growing a stockpile of Cholera patches (x10) | ||
| + | |||
| + | Begin looking for 3D modeling software. | ||
| + | |||
| + | Set up overnights of CRO + DH5alpha for later infection with bacteriophage Lambda. | ||
| + | |||
| + | 10/10 Plate top agar assays of SdiA+/-, infect with bacteriophage Lambda, grow at 37 C. | ||
<br> | <br> | ||
| Line 42: | Line 50: | ||
<font size="4"> '''10/11/13''' </font> | <font size="4"> '''10/11/13''' </font> | ||
| + | Streak singles and start overnights with LOTS of SdiA+/- lysogens, PLATE NEXT TO CHOLERA. PCR SdiA+ from boiled K12 template and from not-boiled K12. | ||
| + | 10/12 Plate SdiA+/- Top Agar Assays with Cholera in the middle (6 SdiA+ lysogens and 6 SdiA- lysogens) | ||
<br> | <br> | ||
| Line 48: | Line 58: | ||
<font size="4"> '''10/14/13''' </font> | <font size="4"> '''10/14/13''' </font> | ||
| + | Failed (contaminated) top agar tests!! Remake overnight cultures, and plate more lysogens (Amber and Arick); PCR clean-up of SdiA+, digest with PST1 and Xba1 | ||
| + | 10/15 Top agar SdiA+/- assays, run SdiA on low-melt gel and ligate | ||
<br> | <br> | ||
| Line 54: | Line 66: | ||
<font size="4"> '''10/16/13''' </font> | <font size="4"> '''10/16/13''' </font> | ||
| + | This time, our top agar lawns were not contaminated, however, we did not see the results we expected! Not one plate had any plaques, not | ||
<br> | <br> | ||
Revision as of 22:27, 21 October 2013
| ||
|
|
10/9/2013 Set up overnights of SdiA+/- strains for later infection with bacteriophage Lambda. Lysogens will plated next to Cholera to prove that SdiA is necessary for lytic induction of bacteriophage Lambda. Begin growing a stockpile of Cholera patches (x10) Begin looking for 3D modeling software. Set up overnights of CRO + DH5alpha for later infection with bacteriophage Lambda. 10/10 Plate top agar assays of SdiA+/-, infect with bacteriophage Lambda, grow at 37 C.
10/11/13 Streak singles and start overnights with LOTS of SdiA+/- lysogens, PLATE NEXT TO CHOLERA. PCR SdiA+ from boiled K12 template and from not-boiled K12. 10/12 Plate SdiA+/- Top Agar Assays with Cholera in the middle (6 SdiA+ lysogens and 6 SdiA- lysogens)
10/14/13 Failed (contaminated) top agar tests!! Remake overnight cultures, and plate more lysogens (Amber and Arick); PCR clean-up of SdiA+, digest with PST1 and Xba1 10/15 Top agar SdiA+/- assays, run SdiA on low-melt gel and ligate
10/16/13 This time, our top agar lawns were not contaminated, however, we did not see the results we expected! Not one plate had any plaques, not
10/18/13
10/21/13 
10/23/13
10/25/13
|
|
