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Team:BYU Provo/Notebook/LargePhage/Summerexp/Period2/Dailylog
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===7/17/13=== | ===7/17/13=== | ||
Today I did another round of mutagenesis using the lysis inhibition technique. I did two flasks. The first flask had 20 ug/mL of adenine which was added with the bacteria and 25 mL of LB broth; the second flask had 40 ug/mL of adenine added with the bacteria and 25 mL of LB broth. Both flasks incubated at 37C on the shaker for 3.5 hrs until slightly turbid. I then added 200 ug/mL of bromodeoxyuridine, 20 ug/mL of uracil, and .5 mL of phage to flask one; I added 400 ug/mL of bromodeoxyuridine, 40 ug/mL of uracil, and .5 mL of phage to flask two. They incubated in the same location for 3 hours. | Today I did another round of mutagenesis using the lysis inhibition technique. I did two flasks. The first flask had 20 ug/mL of adenine which was added with the bacteria and 25 mL of LB broth; the second flask had 40 ug/mL of adenine added with the bacteria and 25 mL of LB broth. Both flasks incubated at 37C on the shaker for 3.5 hrs until slightly turbid. I then added 200 ug/mL of bromodeoxyuridine, 20 ug/mL of uracil, and .5 mL of phage to flask one; I added 400 ug/mL of bromodeoxyuridine, 40 ug/mL of uracil, and .5 mL of phage to flask two. They incubated in the same location for 3 hours. | ||
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| + | The purification group also centrifuged (in CsCl gradient) the T4 stock and the T4 LB mutagenesis / lysis inhibition sample. Hopefully they will return it to us separated into individual fractions. | ||
===7/19/13=== | ===7/19/13=== | ||
Revision as of 21:00, 17 July 2013
Contents |
July
7/17/13
Today I did another round of mutagenesis using the lysis inhibition technique. I did two flasks. The first flask had 20 ug/mL of adenine which was added with the bacteria and 25 mL of LB broth; the second flask had 40 ug/mL of adenine added with the bacteria and 25 mL of LB broth. Both flasks incubated at 37C on the shaker for 3.5 hrs until slightly turbid. I then added 200 ug/mL of bromodeoxyuridine, 20 ug/mL of uracil, and .5 mL of phage to flask one; I added 400 ug/mL of bromodeoxyuridine, 40 ug/mL of uracil, and .5 mL of phage to flask two. They incubated in the same location for 3 hours.
The purification group also centrifuged (in CsCl gradient) the T4 stock and the T4 LB mutagenesis / lysis inhibition sample. Hopefully they will return it to us separated into individual fractions.
7/19/13
7/22/13
7/24/13
7/26/13
7/29/13
7/31/13
