BioBrick
Favorite Tianjin 2013 iGEM Team Parts
| Name |
Type |
Description |
Designer |
Length |
| Bba_K1020005 |
Composite |
Alkane Marker--Device for detection of various alkanes |
Boxuan Zeng
Yue Liu |
2146bp |
| Bba_K1020012 |
Composite |
Alkane Selector for intracellular alkane production |
Boxuan Zeng
Yue Liu |
2662bp |
Tianjin 2013 iGEM Team Parts Sandbox
| Name |
Type |
Description |
Designer |
Length |
| Bba_K1020001 |
Reporter |
PalkM promoter with reporter gene |
Boxuan Zeng
Yue Liu |
1013bp |
| Bba_K1020002 |
Coding |
alkane regulatory protein ALKR |
Boxuan Zeng |
927bp |
| Bba_K1020003 |
Reporter |
AlkR -PSB1C3-Low constitutive |
Yue Liu |
1125bp |
| Bba_K1020004 |
Composite |
Biosensor for alkane detection |
Yue Liu
Su Wang |
2009bp |
| Bba_K1020006 |
Coding |
YbhGFSR |
Boxuan Zeng
Yang Wu |
4971bp |
| Bba_K1020007 |
Composite |
AlkR -PSB1C3-High constitutive |
Yue Liu |
1125bp |
| Bba_K1020008 |
Composite |
alkane producing module |
Boxuan Zeng
Yue Liu |
1809bp |
| Bba_K1020009 |
Composite |
YbhGFSR operon |
Boxuan Zeng |
5034bp |
| Bba_K1020010 |
Coding |
TetA gene under the control of PalkM |
Boxuan Zeng
Shoujie Sun |
1529bp |
| Bba_K1020011 |
Composite |
High expressed Alkane Selector for intracellular alkane production |
Boxuan Zeng
Qingyi Deng |
2662bp |
Others
Results for Agarose Gel Electrophoresis:
Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT
Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT
Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT
Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3
Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3
Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3
Figure 1. The visible color change of the strain cultured in 3ML M9 medium with different induced for 12 hours.
Figure 2. Strain one contains alkane producer part(left),another contains none-producer part (right) .
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