Team:Groningen/Labwork/19 July 2013

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<h2>Inne</h2>
<br> No colonies have grown on the cm plates.
<br> No colonies have grown on the cm plates.
<br> There were supposed to be colonies that contained the GFP biobrick.
<br> There were supposed to be colonies that contained the GFP biobrick.
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'''Sander and Erik-Jan'''
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<h2>Sander and Erik Jan</h2>
<br>  Did a PCR of Silk 2 (strepF-R) and Silk 3 (F-StrepR).
<br>  Did a PCR of Silk 2 (strepF-R) and Silk 3 (F-StrepR).
<br> annealing temperatures of 78 C for silk 2 an 80 C for silk 3
<br> annealing temperatures of 78 C for silk 2 an 80 C for silk 3
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'''Sander'''
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<h2>Sander</h2>
<br> ran restriction analysis of silk 1 (F-R) on 0,8% agarose gel. 90 V for 34 min.
<br> ran restriction analysis of silk 1 (F-R) on 0,8% agarose gel. 90 V for 34 min.
<br> low concentration on gel.
<br> low concentration on gel.
<br> did a ligation reaction on silk 1 with each of the Signal Sequences (MotB, FliZ, EstA and LytB) at 1:1 ratio.
<br> did a ligation reaction on silk 1 with each of the Signal Sequences (MotB, FliZ, EstA and LytB) at 1:1 ratio.
<br> did a PCR of ligation reactions. same protocol as silk 3 above.
<br> did a PCR of ligation reactions. same protocol as silk 3 above.
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  <p>iGEM 2013 Groningen</p>
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Revision as of 12:09, 24 July 2013

Inne


No colonies have grown on the cm plates.
There were supposed to be colonies that contained the GFP biobrick.

Sander and Erik Jan


Did a PCR of Silk 2 (strepF-R) and Silk 3 (F-StrepR).
annealing temperatures of 78 C for silk 2 an 80 C for silk 3

Sander


ran restriction analysis of silk 1 (F-R) on 0,8% agarose gel. 90 V for 34 min.
low concentration on gel.
did a ligation reaction on silk 1 with each of the Signal Sequences (MotB, FliZ, EstA and LytB) at 1:1 ratio.
did a PCR of ligation reactions. same protocol as silk 3 above.

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