Team:BostonU/NotebookML

From 2013.igem.org

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<h2>May</h2>
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<h10>Week of May 12, 2013</h10>
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    <li>Learned about synthetic biology, MoClo, and developed plans for our summer projects, specifically <a href = “https://2013.igem.org/Team:BostonU/HK”>histidine kinase</a> and <a href=”https://2013.igem.org/Team:BostonU/QS”>quorum sensing</a>.</li>
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    <li>Learned about Clotho software suite and learned how to use Eugene, RavenCAD, and Pigeon from other members of the <a href = “http://wiki.bu.edu/ece-cidar/index.php/Main_Page”>CIDAR lab</a>.</li>
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    <li>Familiarized ourselves with protocols and lab etiquette</li>
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    <li>Stocked up on lab supplies (plates, broth, plasmids)</li>
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Revision as of 03:51, 23 July 2013



Characterization Notebook

Goal: Develop a well-characterized library of parts for the MoClo Assembly Method

Subgoals:
  • Assemble level 0 parts needed
    • Constitutive Promoter Characterization Project:
      • J23100_AB, J23113_AB, J23115_AB, J23115_EB, J23116_AB, J23116_EB, J23117_AB, J23118_AB, J23119_AB
      • BCD1_BC, BCD2_BC, BCD8_BC, BCD12_BC, BCD13_BC, BCD16_BC: completed 6/27/13
    • Repressible Promoter Characterization Project:
      • C0012 (LacI), C0062_CD (LuxR), C0071_CD (rhlR_CD), pLacI (R0010_FB)
  • Assemble level 1 circuits for characterization of Constitutive Promoter Library
    • 160 circuits that have different combinations of J231XX promoters and RBS’s
    • 1 set with all promoters + BCD2
    • 1 set with all RBS’s+J23100
  • Assemble level 1 circuits for characterization of Repressible Promoter
  • Run flow cytometry on the promoter characterization circuits
  • Assemble level 2 inverter as an example of the library’s use
  • Switch the backbone in some existing level 1 parts to study how different antibiotics affect the flow cytometry data: completed:6/21/13

May

Week of May 12, 2013
  • Learned about synthetic biology, MoClo, and developed plans for our summer projects, specifically histidine kinase and quorum sensing.
  • Learned about Clotho software suite and learned how to use Eugene, RavenCAD, and Pigeon from other members of the CIDAR lab.
  • Familiarized ourselves with protocols and lab etiquette
  • Stocked up on lab supplies (plates, broth, plasmids)

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