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Team:Groningen/Labwork/25 June 2013
From 2013.igem.org
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Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove. | Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove. | ||
| - | + | <h2>Mirjam and Claudio</h2> | |
<br/>''B. subtilis'' strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for the extraction of genomic DNA is arranged. | <br/>''B. subtilis'' strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for the extraction of genomic DNA is arranged. | ||
| + | |||
| + | <h2>Claudio</h2> | ||
| + | |||
| + | <br>The design for the heat motility sub-project continues. | ||
Revision as of 15:37, 24 July 2013
Mirjam, Chaline and Inne
A couple of media are prepared:
LB medium with agar 1 liter
LB medium divided into 80 ml portions
70% ethanol
2 liter TBE buffer Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove.
Mirjam and Claudio
''B. subtilis'' strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for the extraction of genomic DNA is arranged.
Claudio
The design for the heat motility sub-project continues.
