"
Team:DTU-Denmark/Notebook/26 July 2013
From 2013.igem.org
(Difference between revisions)
(→Lab 208) |
|||
| Line 24: | Line 24: | ||
==Main purpose== | ==Main purpose== | ||
<hr/> | <hr/> | ||
| - | * miniprep of TAT, Sec, and Nir | + | * miniprep of TAT, Sec, and Nir for sequencing |
* LB medium preparation (2L) | * LB medium preparation (2L) | ||
| + | *Pick up new PAO1 cultures | ||
| + | *Make new colony PCR to get Nir from the newly acquired colonies. | ||
==Who was in the lab== | ==Who was in the lab== | ||
<hr/> | <hr/> | ||
| - | + | Kristian, Julia | |
==Procedure== | ==Procedure== | ||
<hr/> | <hr/> | ||
| + | PCRs: | ||
| + | *pZA21::RFP with primer pair 13 58C and 3:00. | ||
| + | *araBAD from K808000 with primer pair 12 61C and 1:00 | ||
| + | *Colony PCR with first PAO1 colony but with a gradient of concentrations from 1x to 500x | ||
| + | *Colony PCR with the 4 new plates of PAO1, concentration x1 | ||
==Results== | ==Results== | ||
<hr/> | <hr/> | ||
| + | See tomorrows gel. | ||
==Conclusion== | ==Conclusion== | ||
| + | Nothing to conclude from todays work. | ||
Navigate to the [[Team:DTU-Denmark/Notebook/25_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/27_July_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/25_July_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/27_July_2013|Next]] Entry | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Revision as of 20:39, 28 July 2013
26 July 2013
Contents |
Lab 115
Main purpose
Who was in the lab
Helen, Kasia
Procedure
Results
Conclusion
Lab 208
Main purpose
- miniprep of TAT, Sec, and Nir for sequencing
- LB medium preparation (2L)
- Pick up new PAO1 cultures
- Make new colony PCR to get Nir from the newly acquired colonies.
Who was in the lab
Kristian, Julia
Procedure
PCRs:
- pZA21::RFP with primer pair 13 58C and 3:00.
- araBAD from K808000 with primer pair 12 61C and 1:00
- Colony PCR with first PAO1 colony but with a gradient of concentrations from 1x to 500x
- Colony PCR with the 4 new plates of PAO1, concentration x1
Results
See tomorrows gel.
Conclusion
Nothing to conclude from todays work. Navigate to the Previous or the Next Entry
