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06/08/13
From 2013.igem.org
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==Running of an agarose gel== | ==Running of an agarose gel== | ||
*Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day. | *Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day. | ||
| - | *expectation-7kb band, confirming the presence of the limonene biobrick (5kb) and chloramphenicol backbone (2kb) | + | *expectation-7kb band, confirming the presence of the limonene biobrick (5kb) and chloramphenicol backbone (2kb) |
| + | [[File:igem_single_dig_060813.jpg]] | ||
==Making chloroamphenicol agar plates== | ==Making chloroamphenicol agar plates== | ||
Revision as of 14:17, 6 August 2013
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Running of an agarose gel
- Running the gel for digests of samples 5.1, 10.1, 10.2 and 10.3 from the previous day.
- expectation-7kb band, confirming the presence of the limonene biobrick (5kb) and chloramphenicol backbone (2kb)
Making chloroamphenicol agar plates
- Making additional plates
- 400ml of agar
- Melt in microwave
- 3mins on low -> shake
- Repeat
- 2mins on low -> shake
- Repeat
- Add 800ul of chloroamphenicol at a concentration of 25 ul/ml
- Pour out onto 20 petri dishes
- Leave to set on bench
- 400ml of agar
