Team:DTU-Denmark/Notebook/7 August 2013

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(Results)
(Who was in the lab)
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==Who was in the lab==
==Who was in the lab==
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Kristian, Gosia, Julia, Henrike
==Procedure==
==Procedure==

Revision as of 13:13, 7 August 2013

7 August 2013

Contents

lab 208


Main purpose


Who was in the lab


Kristian, Gosia, Julia, Henrike

Procedure


Results


Gel on yesterdays PCR

ara, SPL is from yesterdays PCR with 5% DMSO, Nir and the sample numbers is also from yesterday, here the composition can be seen.

  • ara
  • ara
  • SPL
  • SPL
  • Nir 0 MgCl2
  • Nir 1uL MgCl2
  • Nir 5uL MgCl2
  • Nir 20uL MgCl2
  • 12 - Nir2, extraction PCR, primers 42a, 42b, cells in water, plus MgCl2 additive, x7 polymerase
  • 13 - Nir1 with USER primers, primers 39a, 39b, x7, template - Nir purified from gel
  • 14 - Nir1 with USER primers, primers 39a, 39b, x7, template - Nir purified from gel
  • 15 - check Nir1 with primers for NirM+S, primers 11a, 44, template - Nir purified from gel, Phusion polymerase
  • 16 - check Nir1 with primers for NirM+S, primers 11a, 44, template - Nir purified from gel, Phusion polymerase
  • 7 - Nir2, extraction PCR, primers 42a, 42b, cells in water, Phusion polymerase
  • 8 - Nir2, extraction PCR, primers 42a, 42b, cells in water, Phusion polymerase
  • 9 - Nir2, extraction PCR, primers 42a, 42b, cells in water, x7 polymerase
  • 10 - Nir2, extraction PCR, primers 42a, 42b, cells in water, x7 polymerase


not on the gel: 11 - Nir2, extraction PCR, primers 42a, 42b, cells in water, plus MgCl2 additive, Phusion polymerase

2013-08-07 ara nir2.jpg

Decided to purify ara, 12, 7, 8, 9 and 10

Purification gel

  • 1 kb ladder
  • ara
  • ara
  • 12
  • 7
  • 8
  • 9
  • 10
  • 11
  • Nir colony PCR X7
  • 1 kb

Conclusion


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