08/08/13

From 2013.igem.org

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(Created page with "==Making IPTG and chloroamphenicol plates== *400ml of agar **Boil for 3min x2 **Boil for 2min x2 *Cool to 40C *Add 800ul of chloroamphenicol at 25um/ml *Add 400ul IPTG at 0.15um/ml")
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{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
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!align="center"|[[Team:Leicester|Home]]
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!align="center"|[[Team:Leicester/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
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!align="center"|[[Team:Leicester/Project|Project]]
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!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Leicester/Modeling|Modeling]]
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!align="center"|[[Team:Leicester/Notebook|Notebook]]
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!align="center"|[[Team:Leicester/Safety|Safety]]
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!align="center"|[[Team:Leicester/Attributions|Attributions]]
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==Making IPTG and chloroamphenicol plates==
==Making IPTG and chloroamphenicol plates==
*400ml of agar
*400ml of agar

Revision as of 14:58, 8 August 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Making IPTG and chloroamphenicol plates

  • 400ml of agar
    • Boil for 3min x2
    • Boil for 2min x2
  • Cool to 40C
  • Add 800ul of chloroamphenicol at 25um/ml
  • Add 400ul IPTG at 0.15um/ml