09/08/13
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+ | ==Isolating plasmid== | ||
+ | *From overnight culture, took 3ml of culture and centrifuged into pellet of samples 5.1, 10.1, 10.2, 10.3 | ||
+ | *Isolated the plasmid using omega bio-tek Plasmid mini kit I | ||
+ | |||
+ | *Concentrations are shown in the table below | ||
+ | |||
+ | {|border=1 | ||
+ | |Sample||Volume(ul)||Conc.(ng/ul)||260/280||260/230 | ||
+ | |- | ||
+ | | |
Revision as of 14:21, 9 August 2013
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Isolating plasmid
- From overnight culture, took 3ml of culture and centrifuged into pellet of samples 5.1, 10.1, 10.2, 10.3
- Isolated the plasmid using omega bio-tek Plasmid mini kit I
- Concentrations are shown in the table below
Sample | Volume(ul) | Conc.(ng/ul) | 260/280 | 260/230 |