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Team:Paris Bettencourt/Notebook/Trojan Horse/Friday 26th July.html
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Revision as of 12:27, 20 September 2013
Trojan Horse
ASDF26th July
Place your twit here
Infectiveness characterization experiments
- Dilute 1/100 MGZ1, F+ O/N culture in 10mL LB => wait OD(600) = 0.7
- Centrifuge M13K07 4000rpm, 10 minutes
- Retrieve surnageant and filter it with 0.2 filter
- Once OD 0.7 is reached, make 4 tubes
- 1mL MGZ1,F+ + 100ul LB
- 1mL MGZ1,F+ + 100ul surnageant diluted 1/100
- 1mL MGZ1,F+ + 100ul surnageant diluted 1/1000
- 1mL MGZ1,F+ + 100ul surnageant diluted 1/1000
- Incube 37°C for 45 minutes
- Serial dilute the tubes 10^-1, 10^-2, 10^-3, 10^-4, 10^-5, 10^-6
- 4 times 6 tubes with 900uL LB, add 100ul of one previously made tube, mix and transfert 100ul to next tube.
