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Team:BYU Provo/Notebook/SmallPhage/Springexp/Period1/Dailylog
From 2013.igem.org
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- Spot test using stock 5.3 and its dilution series (from 5.4) | - Spot test using stock 5.3 and its dilution series (from 5.4) | ||
| - | : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 | + | : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 Phage Amplification/Purification|5.3 T7 phage amplification/purification]] |
- Started liquid culture for purification team (at around noon) | - Started liquid culture for purification team (at around noon) | ||
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- Started 5.5 amplification from a plaque test | - Started 5.5 amplification from a plaque test | ||
| - | : [[5.5 Amplification from a plaque test]] | + | : [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.5_Amplification_from_a_plaque_test|5.5 Amplification from a plaque test]] |
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<font size="4"> '''5/6/13''' </font> | <font size="4"> '''5/6/13''' </font> | ||
| - | - Continued [[5.3 T7 phage amplification/purification]] | + | - Continued [[Team:BYU_Provo/Notebook/SmallPhage/Springexp/Period1/Exp/Team:BYU_Provo/5.3 T7 phage selection method test|5.3 T7 phage amplification/purification]] |
- Performed liquid culture phage concentration test | - Performed liquid culture phage concentration test | ||
Revision as of 19:53, 27 May 2013
| Small Phage Spring Notebook: April 29 - May 12 Daily Log
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5/1/13 - Commencement of spring!!! - Discussed goals and outlined plans for spring term
5/2/13 - Designed primers for amplifying and sequencing phage capsid protein - Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly
5/3/13 - Performed agar test, focusing primarily on ×8 - Processed phage amplification
5/4/13 - Performed dilution series using stock 5.3 (-1 through -11) - Started two 5mL overnights of BL21
5/5/13 - Spot test using stock 5.3 and its dilution series (from 5.4) - Started liquid culture for purification team (at around noon)
- Started 5.5 amplification from a plaque test
5/6/13 - Continued 5.3 T7 phage amplification/purification - Performed liquid culture phage concentration test
5/7/13 - Started two 5mL of E coli BL21 overnight - Designed procedure for applying mutagen and selecting for T7
5/8/13 - Went over procedure for applying mutagen and PCR with Dr. Grose - Performed spot tests under 5.6 T7+ Liquid Culture Phage Concentration Test - Started two 5mL BL21 overnights - Learned how to create LB plates
5/9/13 - Practiced with ×6 and ×8 top agar - Started 5.9 T7+ Liquid Culture Phage Concentration Test #2 - Discussed plans and schedule for next week.
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