Team:Groningen/Labwork/17 August 2013

From 2013.igem.org

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The gel was loaded in duplo because there was to much sample to fit in one well
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Revision as of 14:56, 17 August 2013

Inne

For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli.

The hy-spank GFP e.coli plates grew colonies, 4 single colonies will be picked and inoculated.

Performed a restriction digest of ms2 samples 1 till 3.
0.8% gel was loaded as following.
well 1 well 2 well 3 well 4 well 5 well 6 well 7 well 8 well 9 well 10
1kb DNA ruler ms2 1 ms2 2 ms2 3 uncut plasmid ms2 1 1kb DNA ruler ms2 1 ms2 2 ms2 3 uncut plasmid ms2 1
The gel was loaded in duplo because there was to much sample to fit in one well