Team:KU Leuven/Project/Aphid Background
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<img src="https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg" alt="Aphid colors"/> | <img src="https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg" alt="Aphid colors"/> | ||
<p>Figure 1ǀ Red, yellow-green and green pea aphids.</p> | <p>Figure 1ǀ Red, yellow-green and green pea aphids.</p> | ||
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+ | <!-- Methylsalicylate --> | ||
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+ | <h3><br/></h3> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg" alt="Aphid colors"/> | ||
+ | <p>Figure 1ǀ Red, yellow-green and green pea aphids.</p> | ||
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+ | <h3>Methylsalicylate</h3> | ||
+ | <p align="justify">When plants are attacked by herbivore insects, they emit a volatile signal that is dependent on the type of insect (Vlot et al, 2008). Herbivore-induced plant volatiles mediate relationships between plants and insects throught the attraction of natural enemies and the repulsion of herbivores (Turlings and Ton, 2006). Manipulation of herbivore-induced plant volatiles can be used to concentrate and increase populations of natural enemies in a specific place, such as crop fields, or to repel pests from crop plants (Khan et al, 2008). Methyl salicylate (MeS) is a phenolic compound that is released when plants are attacked by herbivore insects (Reymond and Farmer, 1998). Upon the activation of the salicylic acid pathway, liquid MeS increases in concentration within a plant, the volatile form is subsequently released in high concentrations (Vlot et al, 2008). Furthermore, MeS itself also induces the salicylic acid pathway and can therefore elicit responses in neighboring healty plants (Heidel and Baldwin, 2004). MeS is also involved in plant defense, it functions as an activator of the defense-related genes. | ||
+ | </p> | ||
</div> | </div> | ||
</div> | </div> |
Revision as of 14:31, 30 August 2013
Secret garden
Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!
- A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
- For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
- We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?
Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!
![tree](https://static.igem.org/mediawiki/2013/a/ac/Tree.png)
![ladybugcartoon](https://static.igem.org/mediawiki/2013/6/64/Ladybugcartoon.png)
Aphid Biology 101
![Aphid colors](https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg)
Figure 1ǀ Red, yellow-green and green pea aphids.
Synopsis
During a long period of co-evolution, plants and aphids have established a complex interaction. On the one hand, aphids have established complex life cycles involving extensive phenotype plasticity with rapid population growth (Blackman and Eastop, 1984) and a very short generation time (Morrison and Peairs, 1998). On the other hand, plants have also evolved delicate and complicated defence systems comprising constitutive defence traits and defence pathways induced upon aphid attack (Chen 2008). Breeders and growers are still struggling to find an efficient strategy for aphid control in major crop plants, as the damage can run in the millions. The KU Leuven iGEM project has an answer to this problem. Our project consists of a genetically engineered bacteria that can reduce the aphid damage on a sustainable way by hacking into their communication system.
Basic aphid biology
Aphids are an extremely successful group of insects which occurs throughout the world, with the greatest number of species in the temperate regions: Europe, North America and Asia (Dixon et al, 1987). As individuals they are small and inconspicuous. However, they can become so numerous that they can damage whole fields or orchards. The complex life cycles and polymorphism enable them to exploit their host plants and respond to every alteration of their environment to a high degree (Biology of Aphids, Dixon, 1973). Aphids are small, mostly soft-bodied insects of the super family Aphidoidea (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn). Their body can be green, black brown, pink or almost colourless (Biology of aphids, Dixon, 1973). About 4400 species of 10 families are known and around 10% of these species are serious pests for agriculture and forestry (Dedryver et al, 2010). Annual worldwide crop losses due to aphids are estimated at hundreds of millions of dollars (Morrison and Peairs 1998).
![Aphid colors](https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg)
Figure 1ǀ Red, yellow-green and green pea aphids.
![Aphid colors](https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg)
Figure 1ǀ Red, yellow-green and green pea aphids.
How aphids feed on plants
The head of aphids is pointed downward for feeding. They use modified mouth parts, stylets, to obtain their food by sucking plant juices (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn). Aphids passively feed on phloem sap of the plants. Once a phloem vessel, which is under high pressure, is punctured, the sap is forced into the aphid’s food canal. Phloem sap is rich in sugars, but poor in amino acids, which are essential for growth. Therefore, aphids ingest a very large amount of food in order to acquire sufficient proteins. The residual solution of digested food is ejected into the exterior in the form of a droplet of honeydew (Biology of Aphids, Dixon, 1973). This sugary liquid is produced through a ‘tail’ at the rear end of the aphid, called a cauda (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn). Most aphids also have paired tubular structures found at the end of the abdomen, called cornicles. Through these cornicles, aphids excrete droplets of a quick-hardening defensive fluid containing triacylglycerols, the cornicle wax. Other defensive compounds, like alarm pheromones, can also be secreted by the cornicles (aphid, McGraw-Hill Encyclopedia of Science and Technology).
Life cycles
Aphids display a diverse range of complicated life cycles (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn). Many aphid species are monophagous, they feed on only one plant species. The economically important aphids tend to have a wider host range and are polyphagous (Blackman and Eastop, 1985). They have a primary woody plant during winter and a secondary herbaceous plant in the summer (Dixon et al, 1994). In addition, aphid species have evolved a wide range of annual cycles and adaptive mechanisms that can vary between and within species (Biology of aphids, Dixon, 1973). Most aphids are parthenogenetic and viviparous for most of the year, but are also capable of sexual reproduction with the production of eggs during the summer. The annual cycle generally (97%) includes a single sexual generation. These mating females (oviparae) lay eggs, all of which are female. On hatching, each egg gives rise to a wingless offspring that gives birth parthenogenetically to further parhenogenetic females (viviparae). These may be winged or wingless, in response to environmental conditions (biology of aphids, Dixon, 1973). Crowding is a particularly strong stimulus to develop aphids of the winged, dispersal stage. Other external factors also play a role in the morph determination: food availability and quality, predators, photoperiod, time of the year and so on. In conclusion, aphids can rapidly exploit agricultural landscapes because of their high reproductive potential, dispersal capacities, adaptability to local environment, short generation time and feed on an endless source of plants (Dedryver et al, 2010).
![The aphid host](https://static.igem.org/mediawiki/2013/3/31/Grafiek_host_aphid2.png)
Figure 3ǀSelection of host by aphid.
![Life cycle](https://static.igem.org/mediawiki/2013/f/fa/Life_cycle3.png)
Figure 4ǀLife cycle of aphids.
![Aphid colors](https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg)
Figure 1ǀ Red, yellow-green and green pea aphids.
Damage to plants and solutions
Aphid damage to plants may be caused directly, by nutrient drain, or indirectly by several mechanisms. These indirect mechanisms include plant virus transmission through the aphids’ piercing-sucking mouthparts, injection of saliva containing phytotoxic components that have a disastrous effect on plant growth and harmful fungi growth on the aphids’ honeydew hindering photosynthetic activity. Common damage symptoms on plants are decreased growth rates, mottled leaves, yellowing, stunted growth, curled leaves, gall formation and low yields and death (Biology of Aphids, Dixon, 1973). Different methods can be used to control an aphid infestation. For many crops, insecticides provide a simple and effective strategy for aphid control. However, the application of such chemicals is not desirable in the long term, because of the development of insecticide resistance and the potential negative effects on the population dynamics of other beneficial insects (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn). Aphids are vulnerable to many kinds of predators, including ladybird beetles and parasitoid wasps, and parasites and these can also be used to control aphids (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn).
Synopsis
Attraction of insects to plants and other host organisms involves detection of specific semiochemicals (natural signal chemicals mediating changes in behavior and development) or specific rations of these semiochemicals (Pickett et al, 2006). Plants colonized and damaged by herbivorous insects produce a group of volatile organic compounds, herbivore-induced plant volatiles. These compounds act through induced direct and indirect defenses on the herbivore pests (Lou et al, 2006). The direct defenses include chemicals that act as repellents for herbivorous pests. The indirect defenses increase the herbivore mortality through the recruitment of parasitoids and predators (Hatano et al, 2008).
![Aphid colors](https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg)
Figure 1ǀ Red, yellow-green and green pea aphids.
Prey localization
Predatory and parasitoid insects have a specialized sensory nervous system to detect their prey (Hatano et al, 2008). They use volatiles produced by the herbivores, reliable but at low concentration, or those produced by the plant to locate their prey. These latter are easily detectable, but are less reliable. To overcome the reliablility-detecability problem predators and parasitoids focus on the responses on stimuli created by specific interactions between the herbivore and its plant (Vet & Dicke, 1992). In response to an aphid attack, plants modify their volatile emissions and these changes are detected by aphid enemies (Du et al, 1998). Host selection occurs in three phases: habitat localization, host localization and host acceptance (Vinson, 1976). Aphid natural enemies must first locate the aphid habitat, the host plant where aphids are present. Therefore, plant-derived volatiles are used, since evidence of aphid damage is acquired. One of the important herbivore-induced plant volatiles that are used by predators is methyl salicylate (MeS) (Zu & Park, 2005). The secretion of MeS is induced by the feeding of aphids on the plant (Birkett et al, 2000). Following habitat localization, natural enemies use short range physical (colour, shape, movement of aphid) and chemical cues to search for a suitable herbivore on the host plant (Hatano et al, 2008). One of the chemical cues used by natural enemies is aphid honeydew. Mostly, the natural enemies need physical contact with honeydew to change their behavior (Ide et al, 2007). In addition to aphid honeydew, the aphid alarm pheromone, (E)-β-farnesene (EBF) is also an important semiochemicals in aphid localization. It is released in the cornicle secretions of many aphid species (Franscis et al, 2005) to alert surrounding aphids of the presence of natural enemies (Kunert et al, 2005). Detection of these short range chemical cues leads not to the aphid directly, but only indicates the presence, improving prey detection of the predators and parasitoids. Once an aphid is located, natural enemies have to recognize it as a potential prey before they attack it. For host recognition, non volatile chemical cues are important, in particular contact with the cornicle wax on the surface of the aphids. Predators use their antennae or their mouthparts to recognize the prey. Parasitoids use probing to assess host quality before oviposition (Hatano et al, 2008).
β-farnesene
Aphid populations are regulated by natural enemies such as ladybird beetles and parasitoid wasps (World crop pests, 2A, Aphids their biology, natural enemies and control, minks and harrewijn). For many pest species of aphids, avoidance of predators involves the release of an alarm pheromone. (E)-β-Farnesene (EβF), a volatile sesquiterpene, is released from the cornicles on the aphids’ abdomen when attacked by its natural enemies (Dixon 1998). Therefore, EβF functions as a direct repellent and acts as a kairomone (chemical messengers emitted by organisms of one species but benefit members of another species) for attraction of natural enemies of aphids (Hatano et al. 2008).
The high oxidation rate of EβF has hampered the application of EβF to cop plants against aphids (Hille Ris Lambers and Schepers 1978). We get around this problem by using E. coli to produce the aphids’ own alarm pheromone (EβF) and use them right on the plants. Beside using the aphid communication, we also want to attract aphid predators such as ladybugs to make sure the aphids are thoroughly removed.
![Aphid colors](https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg)
Figure 1ǀ Red, yellow-green and green pea aphids.
![Aphid colors](https://static.igem.org/mediawiki/2013/e/ee/Colored_aphids.jpg)
Figure 1ǀ Red, yellow-green and green pea aphids.
Methylsalicylate
When plants are attacked by herbivore insects, they emit a volatile signal that is dependent on the type of insect (Vlot et al, 2008). Herbivore-induced plant volatiles mediate relationships between plants and insects throught the attraction of natural enemies and the repulsion of herbivores (Turlings and Ton, 2006). Manipulation of herbivore-induced plant volatiles can be used to concentrate and increase populations of natural enemies in a specific place, such as crop fields, or to repel pests from crop plants (Khan et al, 2008). Methyl salicylate (MeS) is a phenolic compound that is released when plants are attacked by herbivore insects (Reymond and Farmer, 1998). Upon the activation of the salicylic acid pathway, liquid MeS increases in concentration within a plant, the volatile form is subsequently released in high concentrations (Vlot et al, 2008). Furthermore, MeS itself also induces the salicylic acid pathway and can therefore elicit responses in neighboring healty plants (Heidel and Baldwin, 2004). MeS is also involved in plant defense, it functions as an activator of the defense-related genes.