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02/09/13
From 2013.igem.org
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==Preparation of IPTG and Chloramphenicol plates== | ==Preparation of IPTG and Chloramphenicol plates== | ||
| - | *The IPTG/Chloramphenicol plates that were prepared earlier on [[08/08/13]] for the volatile organic compound (VOC) test were found to be contaminated. | + | *The IPTG/Chloramphenicol plates that were prepared earlier on ([[08/08/13]])for the volatile organic compound (VOC) test were found to be contaminated. |
* Hence new plates had to be made using the below protocol; | * Hence new plates had to be made using the below protocol; | ||
Revision as of 12:02, 2 September 2013
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Preparation of IPTG and Chloramphenicol plates
- The IPTG/Chloramphenicol plates that were prepared earlier on (08/08/13)for the volatile organic compound (VOC) test were found to be contaminated.
- Hence new plates had to be made using the below protocol;
PROTOCOL:
- x2 400ml of already prepared and sterilized agar was obtained from the media kitchen.
- The agar was microwaved using the lowest power level at 2-3 mins intervals until completely melted.
- The x2 400ml agar were then placed in the 37 degrees room to cool.
- After cooling, 800ul of cholramphenicol and 400ul of IPTG were added to one bottle whilst only 800ul of chloramphenicol was added to the second bottle.
- The contents of both agar bottles was then poured into ...
