Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period10/Dailylog

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Phage Purification July - August Notebook: August 1 - August 16 Daily Log

8/7/13

- Performed a cesium chloride gradient purification on T7 mutated phage from the small phage team.

8.7 CsCl Gradient

8/9/13

- We performed dialysis on T7 mutated phage from the cesium chloride gradient on 8.7. Because the gradient only showed one band, we decided to not do an EM as we most likely had mutant phage mixed in with wild type. We discussed a gradient to run in the future that will be able to further identify where the phage band. We decide on a CsCl gradient of concentrations 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, 1.5, 1.6. We hope to be able to identify exactly where the phage bands so that we can make small variations in the gradient at that point. This will allow us to separate mutant phage from wild type.

8/12/13

- Performed a CsCl gradient on the T7 mutant phage from the small phage team. We used the same phage that we used on 8.7.

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-<font size="4"> '''8/2/13''' </font>
-- Performed the spot test in [[Team:BYU Provo/Notebook/SmallPhage/Springexp/Period3/Exp/7.29 Mutagen Concentration Test - Sixth Protocol|7.29 Mutagen Concentration Test - Sixth Protocol]].
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-<font size="4"> '''8/5/13''' </font>
-- Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration.
-- Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration.
-- Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.2 Modeling phage plaque size|8.2 Modeling Phage Plaque Sizes - Experiment 1]].
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