"
05/09/13
From 2013.igem.org
(Difference between revisions)
TanviSinha (Talk | contribs) (→Transformation of pSB1C3/TOD genes) |
TanviSinha (Talk | contribs) |
||
| Line 21: | Line 21: | ||
#Add 950 ul of SOC medium (at room temperature) to the ligation reactions. Incubate for 1.5 hours at 37'C on a shaker. | #Add 950 ul of SOC medium (at room temperature) to the ligation reactions. Incubate for 1.5 hours at 37'C on a shaker. | ||
| - | *The controls for this experiment are the resistance and viability controls. One tube has 50 ul of highly competent cells and SOC medium added to it. | + | *The controls for this experiment are the resistance and viability controls. |
| + | *One tube has 50 ul of highly competent cells and SOC medium added to it. | ||
Revision as of 11:10, 5 September 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Transformation of pSB1C3/TOD genes
- Centrifuge the ligation
- Add 6 ul of each ligation reaction to a sterile 1.5ml tube on ice
- Thaw the cells on ice
- Carefully transfer 50ul of cells to the ligation reaction. Gently flick the tubes and incubate on ice for 20 minutes.
- Heat shock the cells for 45 seconds in a 42'C water bath. Do not shake. Immediately return the tubes to ice for 2 minutes.
- Add 950 ul of SOC medium (at room temperature) to the ligation reactions. Incubate for 1.5 hours at 37'C on a shaker.
*The controls for this experiment are the resistance and viability controls. *One tube has 50 ul of highly competent cells and SOC medium added to it.
