Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period11/Dailylog

From 2013.igem.org

(Difference between revisions)

Revision as of 20:09, 6 September 2013

Phage Purification July - August Notebook: August 17 - August 31 Daily Log

8/19/13

8/21/13

8/23/13

-Prepared a Cesium Chloride Gradient for T4

8.23 CsCl gradient

8/26/13

Reviewed results for the T4 gradient. We are having issues getting the T4 to band. When we remove the gradient after centrifuging we are unable to see any band at all. A spot test reveals phage are found all over the gradient. We have had this same result several times and believe that the cesium chloride we are using could be the factor. Our plan is to do a very broad test and see if we can get the phage to band anywhere.

8/28/13

<< Previous Next >>

@@ Line 56: / Line 56: @@
 <font size="4"> '''8/26/13''' </font>
+Reviewed results for the T4 gradient.  We are having issues getting the T4 to band.  When we remove the gradient after centrifuging we are unable to see any band at all.  A spot test reveals phage are found all over the gradient.  We have had this same result several times and believe that the cesium chloride we are using could be the factor.  Our plan is to do a very broad test and see if we can get the phage to band anywhere.
 <br>