User:ChristopherW

From 2013.igem.org

(Difference between revisions)
Line 54: Line 54:
<script type='text/javascript' src='https://2013.igem.org/Team:TU-Munich/Flot.js?action=raw&ctype=text/js'></script>
<script type='text/javascript' src='https://2013.igem.org/Team:TU-Munich/Flot.js?action=raw&ctype=text/js'></script>
<script>
<script>
-
$(document).ready(function() {var d1 = [[0.5, 0.3], [1.5, 2.8], [2.5, -1.5], [3.5, -3]];var d2 = [[0.5, 0.3], [1.5, 0.8], [2.5, -0.5], [3.5, -1]];var options = { grid: { borderWidth: { top: 0, right: 0, bottom: 0, left: 0 } }, xaxes: [{ min: 0, max: 3, ticks: [[0.5, 'M'], [1.5, 'F'], [2.5, 'A']], tickLength: -2 }], yaxes: [{ ticks: [[0, '0'], [4.5,'hydrophobic'], [-4.5,'hydrohilic']], min: -4.5, max: +4.5 }, { ticks: [[0, '0'], [1,'positively charged'], [-1,'negatively charged']], position: 'right', min: -1, max: 1 }]};$.plot('#hydrophobicity_charge_placeholder',[{ color: 'rgba(151,187,205,1)', data: d1, label: 'Hydrophobicity', lines: { show: true, fill: true }, yaxis: 1}, { color: 'rgba(220,220,220,1)', data: d2, label: 'Charge', lines: { show: true, fill: true }, yaxis: 2}],options );}
+
var d1 = [[0.5, 0.3], [1.5, 2.8], [2.5, -1.5], [3.5, -3]];
 +
var d2 = [[0.5, 0.3], [1.5, 0.8], [2.5, -0.5], [3.5, -1]];
 +
var options = {
 +
grid: {borderWidth: { top: 0, right: 0, bottom: 0, left: 0 } }, xaxes: [{ min: 0, max: 3, ticks: [[0.5, 'M'], [1.5, 'F'], [2.5, 'A']], tickLength: -2 }], yaxes: [{ ticks: [[0, '0'], [4.5,'hydrophobic'], [-4.5,'hydrohilic']], min: -4.5, max: +4.5 }, { ticks: [[0, '0'], [1,'positively charged'], [-1,'negatively charged']], position: 'right', min: -1, max: 1 }]};$.plot('#hydrophobicity_charge_placeholder',[{ color: 'rgba(151,187,205,1)', data: d1, label: 'Hydrophobicity', lines: { show: true, fill: true }, yaxis: 1}, { color: 'rgba(220,220,220,1)', data: d2, label: 'Charge', lines: { show: true, fill: true }, yaxis: 2}],options );
</script>
</script>
</html>
</html>

Revision as of 15:09, 7 September 2013

Protein data table for BioBrick BBa_K801060 automatically created by the BioBrick-AutoAnnotator version 1.0
Nucleotide sequence in RFC 10: (underlined part encodes the protein)
 GTACACAATGCGTCGT ... TTCGAAAAATAA
 ORF from nucleotide position 8 to 1705 (excluding stop-codon)
Amino acid sequence: (RFC25 scars in shown in bold, other sequence features underlined; both given below)
1 
101 
201 
301 
401 
501 		 MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE
FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW
FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL
DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT
ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE
FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK*
Sequence features: (with their position in the amino acid sequence, see the list of supported features)
RFC25 scar (shown in bold): 556 to 557
Strep-tag II: 559 to 566
Amino acid composition:
Ala (A)33 (5.8%)
Arg (R)25 (4.4%)
Asn (N)27 (4.8%)
Asp (D)34 (6.0%)
Cys (C)4 (0.7%)
Gln (Q)24 (4.2%)
Glu (E)48 (8.5%)
Gly (G)29 (5.1%)
His (H)18 (3.2%)
Ile (I)39 (6.9%)
Leu (L)64 (11.3%)
Lys (K)36 (6.4%)
Met (M)16 (2.8%)
Phe (F)28 (4.9%)
Pro (P)17 (3.0%)
Ser (S)33 (5.8%)
Thr (T)26 (4.6%)
Trp (W)14 (2.5%)
Tyr (Y)27 (4.8%)
Val (V)24 (4.2%)
Amino acid counting
Total number:566
Positively charged (Arg+Lys):61 (10.8%)
Negatively charged (Asp+Glu):82 (14.5%)
Aromatic (Phe+His+Try+Tyr):87 (15.4%)
Biochemical parameters
Atomic composition:C3002H4586N778O868S20
Molecular mass [Da]:66105.3
Theoretical pI:5.38
Extinction coefficient at 280 nm [M-1 cm-1]:117230 / 117480 (all Cys red/ox)
Codon usage
Organism:E. coliB. subtilisS. cerevisiaeA. thalianaP. patensMammals
Codon quality (CAI):good (0.71)good (0.75)good (0.69)good (0.78)codon usage physcogood (0.68)
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation.
If you have any questions, comments or suggestions, please leave us a comment.

Protein data table for BioBrick BBa_K801060 automatically created by the BioBrick-AutoAnnotator version 1.0
Nucleotide sequence in RFC 10: (underlined part encodes the protein)
 GTACACAATGCGTCGT ... TTCGAAAAATAA
 ORF from nucleotide position 8 to 1705 (excluding stop-codon)
Amino acid sequence: (RFC25 scars in shown in bold, other sequence features underlined; both given below)
1 
101 
201 
301 
401 
501 		MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE
FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW
FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL
DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT
ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE
FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK*
Sequence features: (with their position in the amino acid sequence, see the list of supported features)
RFC25 scar (shown in bold): 556 to 557
Strep-tag II: 559 to 566
Amino acid composition:
Ala (A)33 (5.8%)
Arg (R)25 (4.4%)
Asn (N)27 (4.8%)
Asp (D)34 (6.0%)
Cys (C)4 (0.7%)
Gln (Q)24 (4.2%)
Glu (E)48 (8.5%)
Gly (G)29 (5.1%)
His (H)18 (3.2%)
Ile (I)39 (6.9%)
Leu (L)64 (11.3%)
Lys (K)36 (6.4%)
Met (M)16 (2.8%)
Phe (F)28 (4.9%)
Pro (P)17 (3.0%)
Ser (S)33 (5.8%)
Thr (T)26 (4.6%)
Trp (W)14 (2.5%)
Tyr (Y)27 (4.8%)
Val (V)24 (4.2%)
Amino acid counting
Total number:566
Positively charged (Arg+Lys):61 (10.8%)
Negatively charged (Asp+Glu):82 (14.5%)
Aromatic (Phe+His+Try+Tyr):87 (15.4%)
Biochemical parameters
Atomic composition:C3002H4586N778O868S20
Molecular mass [Da]:66105.3
Theoretical pI:5.38
Extinction coefficient at 280 nm [M-1 cm-1]:117230 / 117480 (all Cys red/ox)
Hydrophobicity and charge plot
Codon usage
Organism:E. coliB. subtilisS. cerevisiaeA. thalianaP. patensMammals
Codon quality (CAI):good (0.71)good (0.75)good (0.69)good (0.78)excellent (0.80)good (0.68)
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation.
If you have any questions, comments or suggestions, please leave us a comment.

Retrieved from "http://2013.igem.org/User:ChristopherW"