Team:AITM-Nepal/Human practise

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<a href="https://2013.igem.org/Team:AITM-Nepal/Safety">Safety</a>
<a href="https://2013.igem.org/Team:AITM-Nepal/Safety">Safety</a>
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                                <a href="https://2013.igem.org/Team:AITM-Nepal/Human_practise">Human Practice</a>
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<a href="https://2013.igem.org/Team:AITM-Nepal/Attributions">Attributions</a>
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<h2><b><font color="white">Our Project:</font></b></h2>
 
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<h3><b><font color="white">ImmunEcoli</font></b></h3>
 
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<font color="white">This is the first approach for Synthetic Biology from Nepal. We are a team of Undergrads of Biotechnology from Asian Institute of Technology and Management. This year we are trying to engineer ''Escherichia coli'' which will be used as a carrier and immune modulation point using Outer Membrane Vesicle (OMVs). ''Escherichia coli'', being a model organism, is easier to work with , especially for the purpose of genetically engineered realm.
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<font size=6 color="black">Human Practice:</font><br>
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<br>
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The first program conducted by the human practice team was in Little Angel’s College, Hattiban (Lalitpur) for the intermediate level that is +2 students .A huge number of students participated in program which was about 160.The program contained presentation on synthetic biology, its application and future plans in Nepal. Different section of program topic was presented by different member of the team. As it was the first program conducted by the team , they  gave the general concept  of the synthetic biology and iGEM project .During the program it was found that many of the students were not familiar to the subject biotechnology and  synthetic biology. Team gave the overview of their project. Presentors were well known about their topic. Though there were different sub parts regarding synthetic biology during presentation, some of them were enthralling and impressive. It attracted the student very much. Mosty the section containing interactive pictures and cartoon images attracted the students and they paid more attention to the particular section comparatively to other section. Different examples related to different areas of synthetic biology were included so that it made the students to catch the information easily .The team were determined to gave their 100% effort to distribute the information regarding the synthetic biology and its different areas.
 +
During the questionnaire round ,most  the student were shy to ask their question in front of the mass  but some Students arose their questions regarding the scope of synthetic biology. Synthetic biology's application on agricultural aspect was thoroughly described by team members and understood by the students.
 +
Overall the presentation was good as it was a first human practice approach by the team  but there  were few problems which can be overlook for the presenters of the first presentation.
 +
Synthetic biology and research oriented program courses  are not currently included in curriculum of Nepal .Thus it is recommended  that  this  sort  of program  must be included in the curriculum so that student will gain knowledge  and  acquire  habit of doing research work .
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Canonical small interfering RNA (siRNA) duplexes are potent activators of the mammalian innate immune system. The induction of innate immunity by siRNA is dependent on siRNA structure and sequence, method of delivery, and cell type. The delivery of siRNA in a packaged outer membrane vesicle of gram negative bacteria is the theme of our work. The toll like receptor-7/8 activation by siRNA in order to boost the production of Interferon type -1 molecules to inhibit the viral and outer membrane LPS structure to activate Toll like receptor -4 to inhibit bacterial pathogens is the objective of this work. The delivery is made dependent on the peptide fragment which mediated the fusogenic mechanism so as to escape the endosomal compartment once endocytosed inside host(mamalian) cell. Thus freeing the siRNA to silence the myD88 transcript in host cytoplasm making RISC complex and hence, activating TLR-7/8 in endosomal membrane formerly.
 
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Contact us: aitmnepal2013@gmail.com
 
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Revision as of 05:47, 8 September 2013

Human Practice <style> #catlinks,#top-section,#footer-box,#top { display:none; } body{ background-image:url('https://static.igem.org/mediawiki/2013/7/76/Back.jpg'); background-attachment:fixed; background-size:100%; } #p-logo { display:none; } #content { background:#aaaaaa; } #access{ background: linear-gradient(#252525, #0A0A0A) repeat scroll 0 0 transparent; box-shadow: 0 1px 2px rgba(0, 0, 0, 0.4); clear: both; display: block; float: left; margin: auto; margin-bottom:10px; width: 100%; overflow:hidden; } #access ul { font-size: 13px; list-style: none outside none; padding-left: 0; } #access li { float: left; margin-left:5px; position: relative; list-style:none; } #access a{ color: #EEEEEE; display: block; line-height: 3.333em; padding-right:9px; padding-left:9px; text-decoration: none; } #access li:hover > a{ background: #efefef; background: #f9f9f9; /* Show a solid color for older browsers */ background: -moz-linear-gradient(#f9f9f9, #e5e5e5); background: -o-linear-gradient(#f9f9f9, #e5e5e5); background: -webkit-gradient(linear, 0% 0%, 0% 100%, from(#f9f9f9), to(#e5e5e5)); /* Older webkit syntax */ background: -webkit-linear-gradient(#f9f9f9, #e5e5e5); color: #373737; } #headerbar { width:975; height:150px; margin-bottom:15px; margin:auto; padding-left:10px; } h1 { display:none; } #bodyContent { width:1000px; float:left; } #mainbox { background:rgba(66, 125, 118, 0.8); padding:20px; margin-top:50px; border-radius:50px; } </style> </head> <body> <script src="http://code.jquery.com/jquery-1.10.2.min.js"></script> <script src="http://code.jquery.com/jquery-1.10.2.js"></script> <div id="mainbox"> <div id="headerbar"> <img src="https://static.igem.org/mediawiki/2013/d/d1/Hava1.png" height="145px' style="float:left;"> <a href="https://2013.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2013/e/e3/Wiki1.png" style="float:right;" height="120px"></a> </div> <nav id="access"> <div class="menu"> <ul> <li> <a href="https://2013.igem.org/Team:AITM-Nepal">Home</a> </li> <li> <a href="https://2013.igem.org/Team:AITM-Nepal/Team">Team</a> </li> <li> <a href="https://igem.org/Team.cgi?year=2013&team_name=AITM-Nepal">Official Team Profile</a> </li> <li> <a href="https://2013.igem.org/Team:AITM-Nepal/Project">Project</a> </li> <li> <a href="https://2013.igem.org/Team:AITM-Nepal/Parts">Parts Submitted To Registry</a> </li> <li> <a href="https://2013.igem.org/Team:AITM-Nepal/Modeling">Modeling</a> </li> <li> <a href="https://2013.igem.org/Team:AITM-Nepal/Notebook">Notebook</a> </li> <li> <a href="https://2013.igem.org/Team:AITM-Nepal/Safety">Safety</a> </li> <a href="https://2013.igem.org/Team:AITM-Nepal/Human_practise">Human Practice</a> </li> <li> <a href="https://2013.igem.org/Team:AITM-Nepal/Attributions">Attributions</a> </li> </ul> </div> </nav> <font size=6 color="black">Human Practice:</font><br> <br> The first program conducted by the human practice team was in Little Angel’s College, Hattiban (Lalitpur) for the intermediate level that is +2 students .A huge number of students participated in program which was about 160.The program contained presentation on synthetic biology, its application and future plans in Nepal. Different section of program topic was presented by different member of the team. As it was the first program conducted by the team , they gave the general concept of the synthetic biology and iGEM project .During the program it was found that many of the students were not familiar to the subject biotechnology and synthetic biology. Team gave the overview of their project. Presentors were well known about their topic. Though there were different sub parts regarding synthetic biology during presentation, some of them were enthralling and impressive. It attracted the student very much. Mosty the section containing interactive pictures and cartoon images attracted the students and they paid more attention to the particular section comparatively to other section. Different examples related to different areas of synthetic biology were included so that it made the students to catch the information easily .The team were determined to gave their 100% effort to distribute the information regarding the synthetic biology and its different areas. During the questionnaire round ,most the student were shy to ask their question in front of the mass but some Students arose their questions regarding the scope of synthetic biology. Synthetic biology's application on agricultural aspect was thoroughly described by team members and understood by the students. Overall the presentation was good as it was a first human practice approach by the team but there were few problems which can be overlook for the presenters of the first presentation. Synthetic biology and research oriented program courses are not currently included in curriculum of Nepal .Thus it is recommended that this sort of program must be included in the curriculum so that student will gain knowledge and acquire habit of doing research work . </div> </p><div class="printfooter"> Retrieved from "<a href="http://2013.igem.org/Team:AITM-Nepal/Human_practise">http://2013.igem.org/Team:AITM-Nepal/Human_practise</a>"</div> <div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content --> <div class="visualClear"></div> </div> </div> <!-- PAGE FOOTER -- ITEMS FROM COLUMN ! 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