Team:BYU Provo/Notebook/Cholera - Detection/Winterexp/Period3/Dailylog
From 2013.igem.org
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Revision as of 20:26, 5 June 2013
Small Phage March - April Notebook: April 1 - April 14 Daily Log
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4/1/13 - Presentation day!
4/3/13 - Learned about how to start an overnight liquid culture for host bacteria - Learned about how to create bacterial glycerol stock - Made fresh LB and concentrated top agar stock
4/4/13 - T7 arrived - Overnight liquid culture for bacterial host started by Dr. Grose
4/5/13 - Background research on phiX174: the only phage we have in stock - Performed 3.20 Phage Viability Test
3/21/13 - Checked up on results for 3.20 Phage Viability Test
3/22/13 - Discussed results from tittering experiment (preliminary experiment 1)
- Discussed step of attack with Dr. Grose
- Sequencing will be for individual genes to cut down cost
- Learnt about Mega5 to compare genome and protein sequence
3/25/13 - Reported on past week and plans for this week
- Start working on designing our site directed mutagenesis
- Research into in-vitro assembly vs direct mutation of phage genome
3/27/13 - More research on genome of enterobacteria phage
- Outlined protocol for producing stock top agar
3/29/13 - Worked on our first team presentation.
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