Team:BYU Provo/Notebook/Phage Purification/Springexp/Period3/Dailylog
From 2013.igem.org
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<font size="4"> '''6/3/13''' </font> | <font size="4"> '''6/3/13''' </font> | ||
- | - | + | - Ran the CsCl purification step. |
:[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]] | :[[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]] | ||
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<font size="4"> '''6/5/13''' </font> | <font size="4"> '''6/5/13''' </font> | ||
- | - | + | - Extracted the phage from [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/CsClGradientPhagePurification|6.3 CsCl Gradient Phage Purification]] using a syringe and a needle. |
+ | : Only extracted T4 because the T7 gradient was destroyed in the refrigerator. | ||
+ | : Extracted the T4 band as well as debris from the bottom as the gradient was partially destroyed. | ||
+ | : Placed these in the refrigerator. | ||
+ | |||
+ | - Prepared stocks of bacteria for future use. | ||
<br> | <br> |
Revision as of 23:34, 5 June 2013
Phage Purification May - June Notebook: May 27 - June 9 Daily Log
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5/29/13 - Completed the second part of PEG purification.
5/31/13 - We worked on setting up our notebook online.
6/3/13 - Ran the CsCl purification step. - Next class we will attempt extracting the phage from the gradient.
6/5/13 - Extracted the phage from 6.3 CsCl Gradient Phage Purification using a syringe and a needle.
- Prepared stocks of bacteria for future use.
3/20/13
3/22/13
3/25/13
3/27/13
3/29/13
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