"
Team:Wageningen UR/Flux balance analysis
From 2013.igem.org
(Difference between revisions)
Karrenbelt (Talk | contribs) |
Karrenbelt (Talk | contribs) |
||
| Line 14: | Line 14: | ||
<h2>Introduction</h2> | <h2>Introduction</h2> | ||
| - | <p>As a proof of concept lovastatin has been chosen and its production in | + | <p>As a proof of concept of our modular domain approach lovastatin has been chosen and its production in several Aspergilli will be modeled. |
| - | + | ||
To develop and investigate mathematical models of metabolic processes is one of the primary challenges in systems biology. To investigate the potential of lovastatin production, the current metabolic model of A. nigerhas to be expanded. The COBRA toolbox facilitates easy input of the metabolic model in SBML to perform these calculations in MATLAB. Once the model has been expanded flux balance analysis allows for a genome-scale approach. OptKnock can be used to determine which gene knockouts should increase the metabolic flux towards lovastatin. | To develop and investigate mathematical models of metabolic processes is one of the primary challenges in systems biology. To investigate the potential of lovastatin production, the current metabolic model of A. nigerhas to be expanded. The COBRA toolbox facilitates easy input of the metabolic model in SBML to perform these calculations in MATLAB. Once the model has been expanded flux balance analysis allows for a genome-scale approach. OptKnock can be used to determine which gene knockouts should increase the metabolic flux towards lovastatin. | ||
</p> | </p> | ||
Revision as of 10:17, 10 September 2013
- Safety introduction
- General safety
- Fungi-related safety
- Biosafety Regulation
- Safety Improvement Suggestions
- Safety of the Application
Metabolic modeling
Metabolic modeling
of lovastatin biosynthesis in Aspergilli
Introduction
As a proof of concept of our modular domain approach lovastatin has been chosen and its production in several Aspergilli will be modeled. To develop and investigate mathematical models of metabolic processes is one of the primary challenges in systems biology. To investigate the potential of lovastatin production, the current metabolic model of A. nigerhas to be expanded. The COBRA toolbox facilitates easy input of the metabolic model in SBML to perform these calculations in MATLAB. Once the model has been expanded flux balance analysis allows for a genome-scale approach. OptKnock can be used to determine which gene knockouts should increase the metabolic flux towards lovastatin.
Rationale
Aim
Approach
Research Methods
