Team:BYU Provo/Notebook/Phage Purification/Summerexp/Period1/Exp/7.3 EM Phage Preparation

(Difference between revisions)

Revision as of 20:36, 13 September 2013

Phage Purification July - August Notebook: Experiments

7.3 EM Phage Preparation

I) Purpose

Prepare phage so they can be viewed through an electron microscope.

II) Expected Outcome

Visible phage with capsids and tails.

III) Reagants Used

300 mesh copper grid

2% phosphotungstic acid

filter paper

petri dish

T4 lysate

parafilm

IV) Actual Procedure

Place parafilm in the petri dish and put a drop of T4 lysate and a drop of 2% phosphotungstic acid on the parafilm.

Place the 300 grid mesh copper grid on the T4 lysate drop and leave for 20 minutes.

Remove the grid and place on the 2% phosphotungstic acid drop and leave for 2 minutes.

Remove the grid and dry with filter paper by lightly touching the tip to the grid.

Place grid in the EM grid box.

V) Results

The phage were prepared for an EM.

@@ Line 41: / Line 41: @@
 '''III) Reagants Used'''
-: EM Kit
+: 300 mesh copper grid
+: 2% phosphotungstic acid
+: filter paper
+: petri dish
+: T4 lysate
+: parafilm
 '''IV) Actual Procedure'''
-:
+: Place parafilm in the petri dish and put a drop of T4 lysate and a drop of 2% phosphotungstic acid on the parafilm.
+: Place the 300 grid mesh copper grid on the T4 lysate drop and leave for 20 minutes.
+: Remove the grid and place on the 2% phosphotungstic acid drop and leave for 2 minutes.
+: Remove the grid and dry with filter paper by lightly touching the tip to the grid.
+: Place grid in the EM grid box.
 '''V) Results'''